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首页> 外文期刊>Archives of otolaryngology--head & neck surgery. >Undifferentiated human adipose tissue-derived stromal cells induce mandibular bone healing in rats.
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Undifferentiated human adipose tissue-derived stromal cells induce mandibular bone healing in rats.

机译：未分化的人脂肪组织来源的基质细胞诱导大鼠下颌骨愈合。

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摘要

OBJECTIVE: To test the osteo-regenerative potential of adipose tissue-derived stromal cells (ATSCs), an attractive human source for tissue engineering, in a rat model of mandibular defect. Human dermal fibroblasts (HDFs) were used as a differentiated cellular control in the study. DESIGN: The ATSCs and HDFs were isolated from human lipoaspirate and skin biopsy specimens, respectively. Cells were characterized in vitro and then adsorbed on an osteo-conductive scaffold to be transplanted in a mandibular defect of immunosuppressed rats. Naked unseeded scaffold was used as a negative control. MAIN OUTCOME MEASURES: Bone healing was studied by computerized tomography and histologic analysis after 4, 8, and 12 weeks. RESULTS: Computed tomography showed that undifferentiated ATSCs induced successful bone healing of the mandible defect when transplanted in animals, compared with HDFs and negative controls. Histologic analysis demonstrated that the newly formed tissue in the surgical defect retained the features of compact bone. CONCLUSION: Undifferentiated human ATSCs are suitable for cell-based treatment of mandibular defects, even in the absence of previous osteogenic induction in vitro.
机译：目的：在大鼠下颌骨缺损模型中测试脂肪组织来源的基质细胞（ATSC）的骨再生潜力，该来源是组织工程的有吸引力的人类来源。在研究中，人类皮肤成纤维细胞（HDF）被用作分化细胞对照。设计：ATSCs和HDFs分别从人类抽脂和皮肤活检标本中分离。在体外对细胞进行表征，然后将其吸附在骨传导支架上，以移植到免疫抑制大鼠的下颌缺损中。裸露的裸支架用作阴性对照。主要观察指标：第4、8和12周后，通过计算机断层扫描和组织学分析研究骨愈合。结果：计算机断层扫描显示，与HDF和阴性对照组相比，未分化的ATSC在移植到动物中时可成功治愈下颌骨缺损。组织学分析表明，外科缺损中新形成的组织保留了紧密骨的特征。结论：未分化的人ATSC适用于以细胞为基础的下颌骨缺损治疗，即使以前没有体外成骨诱导作用也是如此。

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