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首页> 外文期刊>Archives of virology >Virulence of infectious hematopoietic necrosis virus and Infectious pancreatic necrosis virus coinfection in rainbow trout ( Oncorhynchus mykiss) and nucleotide sequence analysis of the IHNV glycoprotein gene.
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Virulence of infectious hematopoietic necrosis virus and Infectious pancreatic necrosis virus coinfection in rainbow trout ( Oncorhynchus mykiss) and nucleotide sequence analysis of the IHNV glycoprotein gene.

机译：虹鳟鱼（Oncorhynchus mykiss）的传染性造血坏死病毒和传染性胰腺坏死病毒共感染的毒力以及IHNV糖蛋白基因的核苷酸序列分析。

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摘要

The outcomes of a coinfection of rainbow trout ( Oncorhynchus mykiss) with Infectious hematopoietic necrosis virus (IHNV) strain S46 and Infectious pancreatic necrosis virus (IPNV) strain S46 was determined after waterborne infection. Trout infected with the IHNV/IPNV.S46 sample, (a mixed sample containing equal infectious titers of the viruses) showed 50% less mortality than fish infected with either of the reference viruses alone. Forty-five days after the coinfection, IPNV antigens were detected by flow cytometry in 49 to 63% of the leukocytes from the surviving trout; whereas, only 9-15.6% of the leukocytes expressed IHNV viral antigens. IPNV was easily detected by reverse transcription-polymerase chain reaction (RT-PCR), whereas, for IHNV, a second step of amplification of a 753 bp fragment corresponding to the internal sequences of the IHNV G gene was necessary to optimize viral detection. The sequence of the IHNV gene involved in virulence, the glycoprotein (G) gene, was determined for the IHNV.S46 and compared with other sequences available in the GenBank. Changes found were not located in the antigenic domains of the glycoprotein and were considered not significant.
机译：水传播感染后，确定虹鳟（Oncorhynchus mykiss）与传染性造血坏死病毒（IHNV）菌株S46和传染性胰腺坏死病毒（IPNV）菌株S46共同感染的结果。被IHNV / IPNV.S46样品感染的鳟鱼（混合的样品中含有相同的病毒滴度），其死亡率比仅受任何一种参考病毒感染的鱼类低50％。合并感染后四十五天，通过流式细胞术检测到存活鳟鱼中49％至63％的白细胞中IPNV抗原;而只有9-15.6％的白细胞表达IHNV病毒抗原。 IPNV很容易通过逆转录聚合酶链反应（RT-PCR）进行检测，而对于IHNV，第二步扩增对应于IHNV G基因内部序列的753 bp片段是优化病毒检测所必需的。确定了IHNV.S46涉及毒力的IHNV基因的序列，即糖蛋白（G）基因，并与GenBank中的其他序列进行了比较。发现的变化不在糖蛋白的抗原结构域中，并且被认为是不显着的。

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