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首页> 外文期刊>Archives of virology >Surveillance of pathogens causing gastroenteritis and characterization of norovirus and sapovirus strains in Shenzhen, China, during 2011
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Surveillance of pathogens causing gastroenteritis and characterization of norovirus and sapovirus strains in Shenzhen, China, during 2011

机译：2011年中国深圳引起胃肠炎的病原体监测以及诺如病毒和沙波病毒株的特征

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Viral gastroenteritis is one of the most common diseases in humans, and it is primarily caused by rotaviruses (RVs), astroviruses (AstVs), adenoviruses (AdVs), noroviruses (NoVs), and sapoviruses (SaVs). In this study, we determined the distribution of viral gastroenteritis and human calicivirus (HuCVs) in acute gastroenteritis patients in Shenzhen, China, during 2011. Real-time RT-PCR was used to detect norovirus (NoV), group A rotavirus (RV), adenovirus (AdV), and astrovirus (AstV). From a total of 983 fecal samples, NoV was detected in 210 (21.4 %); RoV in 173 (17.6 %); AstV in 10 (1.0 %); and AdV in 15 (1.5 %). Mixed infections involving two NoVs were found in 21 of the 387 pathogen-positive stool specimens. NoV and SaV genotypes were further tested using RT-PCRs and molecular typing and phylogenetic analysis were then performed based on the ORF1-ORF2 region for NoV and a conserved nucleotide sequence in the capsid gene for SaV. Of the 68 typed strains that were sequenced and genotyped, five were NoV G1 (7.5 %) and 63 were NoV GII (96.6 %). GII strains were clustered into five genotypes, including GII.4 (65.1 %; 36 GII.4 2006b and five GII.4 New Orleans), GII.3 (28.6 %), GII.2 (3.2 %), GII.6 (1.6 %), and GII.1 (1.6 %). While all fecal specimens were tested for SaVs, 15 (1.5 %) were positive, and of these, 12 isolates belonged to G1.2, and the remaining three SaV strains belonged to the SaV GII genogroup. Although various HuCVs were detected in acute gastroenteritis patients, NoV GII.4 2006b was more prevalent than the other HuCVs.
机译：病毒性肠胃炎是人类最常见的疾病之一，主要由轮状病毒（RVs），星状病毒（AstVs），腺病毒（AdVs），诺如病毒（NoVs）和Sapoviruses（SaVs）引起。在这项研究中，我们确定了中国深圳在2011年急性病毒性肠胃炎患者中病毒性肠胃炎和人杯状病毒（HuCVs）的分布。实时荧光定量PCR用于检测诺如病毒（NoV），A组轮状病毒（RV） ，腺病毒（AdV）和星状病毒（AstV）。从总共983份粪便样本中，在210份中检测到NoV（21.4％）; RoV 173（17.6％）; 10的AstV（1.0％）;和AdV占15（1.5％）。在387个病原体阳性粪便样本中，有21个发现了涉及两个NoV的混合感染。使用RT-PCR进一步测试NoV和SaV基因型，然后基于NoV的ORF1-ORF2区和衣壳蛋白的衣壳基因中保守的核苷酸序列进行分子分型和系统发育分析。在经测序和基因分型的68株菌株中，有5株是NoV G1（7.5％），有63株是NoV GII（96.6％）。 GII菌株分为5个基因型，包括GII.4（65.1％; 36 GII.4 2006b和5 GII.4新奥尔良），GII.3（28.6％），GII.2（3.2％），GII.6（ 1.6％）和GII.1（1.6％）。虽然所有粪便标本均进行了SAV检测，但有15例（1.5％）呈阳性，其中12株属于G1.2，其余3株SaV属于SaV GII基因组。尽管在急性胃肠炎患者中检测到多种HuCV，但NoV GII.4 2006b比其他HuCV更为普遍。

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