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首页> 外文期刊>Artificial Organs >Lidocaine/Monoethylglycinexylidide test, galactose elimination test, and sorbitol elimination test for metabolic assessment of liver cell bioreactors.
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Lidocaine/Monoethylglycinexylidide test, galactose elimination test, and sorbitol elimination test for metabolic assessment of liver cell bioreactors.

机译：利多卡因/单乙基甘氨酰己糖苷测试，半乳糖消除测试和山梨醇消除测试用于肝细胞生物反应器的代谢评估。

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摘要

Various metabolic tests were compared for the performance characterization of a liver cell bioreactor as a routine function assessment of cultures in a standby for patient application in clinical studies. Everyday quality assessment (QA) is essential to ensure a continuous level of cellular functional capacity in the development of hepatic progenitor cell expansion systems providing cells for regenerative medicine research; it is also of interest to meet safety requirements in bioartificial extracorporeal liver support systems under clinical evaluation. Quality criteria for the description of bioreactor cultures were developed using primary porcine liver cells as a model. Porcine liver cells isolated by collagenase perfusion with an average of 3 x 10(9) primary cells were used in 39 bioreactors for culture periods up to 33 days. Measurements of monoethylglycinexylidide synthesis and elimination of lidocaine, galactose elimination, and sorbitol elimination proved to be useful for routine QA of primary liver cell cultures. We demonstrate two methods for dispensing test substances, bolus administration and continuous, steady-state administration. Bolus test data were grouped in Standard, Therapy, Infection/Contamination, and Cell-free control groups. Statistical analyses show significant differences among all groups for every test substance. Post hoc comparisons indicated significant differences between Standard and Cell-free groups for all elimination parameters. For continuous tests, results were categorized according to number of culture days and time-dependent changes were analyzed. Continuous administration enables a better view of culture health and the time dependency of cellular function, whereas bolus administration is more flexible. Both procedures can be used to define cell function. Assessment of cellular function and bioreactor quality can contribute significantly to the quality of experimental or clinical studies in the field of hepatic bioreactor development.
机译：比较了各种代谢测试的肝细胞生物反应器的性能表征，将其作为常规备用培养物功能评估，供临床研究中的患者使用。日常质量评估（QA）对于确保为肝祖细胞扩增系统的开发提供连续细胞功能水平至关重要，该系统为再生医学研究提供细胞;在临床评估下满足生物人工体外肝支持系统的安全性要求也很有趣。以原代猪肝细胞为模型，建立了描述生物反应器培养物的质量标准。通过胶原酶灌注分离出的猪肝细胞平均与3 x 10（9）原代细胞一起用于39个生物反应器中，培养时间长达33天。单乙基甘氨酰缩水甘油酯合成和利多卡因消除，半乳糖消除和山梨糖醇消除的测量结果被证明可用于原代肝细胞培养的常规QA。我们演示了两种分配测试物质的方法：推注给药和连续，稳态给药。丸测试数据分为标准，治疗，感染/污染和无细胞对照组。统计分析表明，每种测试物质在所有组之间均存在显着差异。事后比较表明，所有消除参数的标准组和无细胞组之间存在显着差异。对于连续测试，根据培养天数对结果进行分类，并分析时间依赖性变化。连续给药可以更好地了解培养的健康状况和细胞功能的时间依赖性，而推注给药则更加灵活。这两个过程均可用于定义单元格功能。细胞功能和生物反应器质量的评估可以大大有助于肝脏生物反应器开发领域的实验或临床研究的质量。

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