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首页> 外文期刊>Artificial Organs >Removal of Cellular-Type Hemoglobin-Based Oxygen Carrier (Hemoglobin-Vesicles) From Blood Using Centrifugation and Ultrafiltration
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Removal of Cellular-Type Hemoglobin-Based Oxygen Carrier (Hemoglobin-Vesicles) From Blood Using Centrifugation and Ultrafiltration

机译：使用离心和超滤技术去除血液中的细胞型血红蛋白基氧气载体（血红蛋白囊泡）

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The hemoglobin-vesicle (HbV) is an artificial oxygen carrier encapsulating a concentrated hemoglobin solution in a phospholipid vesicle (liposome). During or after transporting oxygen, macrophages capture HbVs in the reticuloendothelial system (RES) with an approximate circulation half-life of 3 days. Animal studies show transient splenohepatomegaly after large doses, but HbVs were completely degraded, and the components were excreted in a few weeks. If a blood substitute is used for emergency use until red blood cell transfusion becomes available or for temporary use such as a priming fluid for an extracorporeal circuit, then one option would be to remove HbVs from the circulating blood without waiting a few weeks for removal by the RES. Using a mixture of beagle dog whole blood and HbV, we tested the separation of HbV using a centrifugal Fresenius cell separator and an ultrafiltration system. The cell separator system separated the layers of blood cell components from the HbV-containing plasma layer by centrifugal force, and then the HbV was removed from plasma phase by the ultrafiltration system. The HbVs (250-280nm) are larger than plasma proteins (<22nm diameter) but smaller than blood cell components (>3μm). The size of HbVs is advantageous to be separated from the original blood components, and the separated blood components can be returned to circulation.
机译：血红蛋白囊泡（HbV）是将浓缩的血红蛋白溶液封装在磷脂囊泡（脂质体）中的人工氧气载体。在运输氧气期间或之后，巨噬细胞会在网状内皮系统（RES）中捕获HbV，循环半衰期约为3天。动物研究显示，大剂量后短暂脾脾肿大，但HbVs完全降解，其成分在几周内被排泄。如果将血液替代品用于紧急用途，直到可以进行红细胞输血或临时使用，例如用于体外循环的灌注液，那么一种选择是从循环血液中去除HbV，而无需等待几周时间通过有。使用比格犬全血和HbV的混合物，我们使用离心费森尤斯细胞分离器和超滤系统测试了HbV的分离。细胞分离器系统通过离心力将血细胞成分的层与含HbV的血浆层分离，然后通过超滤系统从血浆相中去除HbV。 HbV（250-280nm）大于血浆蛋白（直径<22nm），但小于血细胞成分（>3μm）。 HbVs的大小有利于与原始血液成分分离，并且分离出的血液成分可以恢复循环。

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