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首页> 外文期刊>Artificial Organs >Platelet activation in ovines undergoing sham surgery or implant of the second generation PediaFlow pediatric ventricular assist device.
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Platelet activation in ovines undergoing sham surgery or implant of the second generation PediaFlow pediatric ventricular assist device.

机译：假手术或第二代PediaFlow小儿心室辅助设备植入物中绵羊的血小板活化。

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The PediaFlow pediatric ventricular assist device (VAD) is a magnetically levitated turbodynamic pump under development for circulatory support of small children with a targeted flow rate range of 0.3-1.5 L/min. As the design of this device is refined, ensuring high levels of blood biocompatibility is essential. In this study, we characterized platelet activation during the implantation and operation of a second generation prototype of the PediaFlow VAD (PF2) and also performed a series of surgical sham studies to examine purely surgical effects on platelet activation. In addition, a newly available monoclonal antibody was characterized and shown to be capable of quantifying ovine platelet activation. The PF2 was implanted in three chronic ovine experiments of 17, 30, and 70 days, while surgical sham procedures were performed in five ovines with 30-day monitoring. Blood biocompatibility in terms of circulating activated platelets was measured by flow cytometric assays with and without exogenous agonist stimulation. Platelet activation following sham surgery returned to baseline in approximately 2 weeks. Platelets in PF2-implanted ovines returned to baseline activation levels in all three animals and showed an ability to respond to agonist stimulation. Late-term platelet activation was observed in one animal corresponding with unexpected pump stoppages related to a manufacturing defect in the percutaneous cable. The results demonstrated encouraging platelet biocompatibility for the PF2 in that basal platelet activation was achieved early in the pump implant period. Furthermore, this first characterization of the effect of a major cardiothoracic procedure on temporal ovine platelet activation provides comparative data for future cardiovascular device evaluation in the ovine model.
机译：PediaFlow儿科心室辅助设备（VAD）是一种磁悬浮涡轮动力泵，正在开发中，其目标流量范围为0.3-1.5 L / min，可为幼儿提供循环支持。随着设备设计的改进，确保高水平的血液生物相容性至关重要。在这项研究中，我们表征了PediaFlow VAD（PF2）的第二代原型在植入和手术过程中的血小板活化，并进行了一系列手术假手术研究，以检查纯粹的手术对血小板活化的影响。另外，表征了新获得的单克隆抗体，并证明其能够定量羊血小板的活化。 PF2植入了17、30和70天的三个慢性绵羊实验中，而假手术则在五个绵羊中进行了30天的监测。在有和没有外源激动剂刺激的情况下，通过流式细胞术测定血液中的循环活化血小板生物相容性。假手术后的血小板活化在大约2周内恢复到基线。植入PF2的绵羊中的血小板在所有三只动物中均恢复到基线激活水平，并显示出对激动剂刺激的反应能力。在一只动物中观察到较晚的血小板活化，这与与经皮电缆的制造缺陷有关的意外泵停止有关。结果表明，PF2的血小板生物相容性令人鼓舞，因为在泵植入期的早期就实现了基础血小板活化。此外，主要心胸手术对颞部羊膜血小板活化作用的第一个表征为今后在羊模型中评估心血管设备提供了比较数据。

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