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首页> 外文期刊>Asian-Australasian Journal of Animal Sciences >Effect of monosaccharide L-fucose and polysaccharide fucoidan on sperm alpha -L-fucosidase activity and relation to sperm-oocyte interaction in pig.
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Effect of monosaccharide L-fucose and polysaccharide fucoidan on sperm alpha -L-fucosidase activity and relation to sperm-oocyte interaction in pig.

机译：单糖L-岩藻糖和多糖岩藻依聚糖对猪精子α-L-岩藻糖苷酶活性及其与精卵相互作用的影响。

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Carbohydrate-protein interactions are known to be important in gamete interactions. Several evidence indicated that a fucose-containing sulfated polysaccharide fucoidan was potential inhibitor of fertilization in vitro and thus fucose seemed to be part of the recognition signal of gamete interaction in mammals. In recent investigation we found that alpha -L-fucosidase activity was present in boar spermatozoa and it was related to sperm binding to and penetration into zona pellucida (ZP) in vitro. The objective of this study was to determine the effects of monosaccharide L-fucose and polysaccharide fucoidan on sperm alpha -L-fucosidase activity and relation to sperm-oocyte interaction in pig. Results indicated that the activity of sperm alpha -L-fucosidase was largely inhibited (62%) when sperm suspension was treated with monosaccharide L-fucose. It also significantly inhibited the number of sperm binding to ZP (32%) and penetration into zona-intact oocytes (72%), but did not inhibit penetration into zona-free oocytes when fertilization medium-contained L-fucose. The chlorotetracycline (CTC) assessment showed that L-fucose did not affect induction of sperm capacitation and acrosome reaction. In contrast, the activity of sperm alpha -L-fucosidase was not inhibited when sperm suspension was treated with polysaccharide fucoidan but sperm-ZP binding was greatly inhibited (85%) and completely blocked sperm penetration into zona-intact or zona-free oocytes. The CTC assessment showed that fucoidan increased the F pattern and decreased the AR pattern sperm. These results suggested that the different inhibitory mechanisms were present between monosaccharide L-fucose and polysaccharide fucoidan on sperm-oocyte interaction, the inhibition effect of alpha -L-fucose on sperm binding and penetrating into ZP caused sperm alpha -L-fucosidase inhibited by alpha -L-fucose..
机译：已知碳水化合物与蛋白质的相互作用在配子相互作用中很重要。一些证据表明，含岩藻糖的硫酸化多糖岩藻依聚糖是体外受精的潜在抑制剂，因此岩藻糖似乎是哺乳动物配子相互作用识别信号的一部分。在最近的调查中，我们发现公猪的精子中存在α-L-岩藻糖苷酶活性，这与精子在体外与透明带（Zona pellucida）结合和渗透有关。这项研究的目的是确定单糖L-岩藻糖和多糖岩藻依聚糖对猪精子α-L-岩藻糖苷酶活性的影响以及与精子-卵母细胞相互作用的关系。结果表明，当用单糖L-岩藻糖处理精子悬浮液时，精子α-L-岩藻糖苷酶的活性被大大抑制（62％）。它也显着抑制精子与ZP结合的数量（32％）和渗透至完整透明带卵母细胞的渗透率（72％），但当受精培养基中含有L-岩藻糖时，不抑制其渗透进入无透明带卵母细胞。氯四环素（CTC）评估表明L-岩藻糖不影响精子获能和顶体反应的诱导。相反，当用多糖岩藻依聚糖处理精子悬浮液时，精子α-L-岩藻糖苷酶的活性没有受到抑制，但是精子-ZP的结合受到了极大的抑制（85％），并完全阻止了精子渗透进入完整的或无透明带的卵母细胞。 CTC评估显示岩藻依聚糖增加了F型精子，减少了AR型精子。这些结果表明，单糖L-岩藻糖和多糖岩藻依聚糖对精子-卵母细胞相互作用存在不同的抑制机制，α-L-岩藻糖对精子结合和渗透进入ZP的抑制作用导致α被α-L-岩藻糖苷酶抑制。 -L-岩藻糖..

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