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首页> 外文期刊>Asian-Australasian Journal of Animal Sciences >Tissues Expression, Polymorphisms Identification of FcRn Gene and Its Relationship with Serum Classical Swine Fever Virus Antibody Level in Pigs
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Tissues Expression, Polymorphisms Identification of FcRn Gene and Its Relationship with Serum Classical Swine Fever Virus Antibody Level in Pigs

机译：猪FcRn基因的组织表达，多态性鉴定及其与猪经典猪瘟病毒抗体水平的关系

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摘要

Neonatal Fc receptor (FcRn) gene encodes a receptor that binds the Fe region of monomeric immunoglobulin G (IgG) and is responsible for IgG transport and stabilization. In this report, the 8,900 bp porcine FcRn genomic DNA structure was identified and putative FcRn protein included 356 amino acids. Alignment and phylogenetic analysis of the porcine FcRn amino acid sequences with their homologies of other species showed high identity. Tissues expression of FcRn mRNA was detected by real time quantitative polymerase chain reaction (Q-PCR), the results revealed FcRn expressed widely in ten analyzed tissues. One single nucleotide polymorphism (SNP) (HQ026019:g.8526 C>T) in exon6 region of porcine FcRn gene was demonstrated by DNA sequencing analysis. A further analysis of SNP genotypes associated with serum Classical Swine Fever Virus antibody (anti-CSFV) concentration was performed in three pig populations including Large White, Landrace and Songliao Black pig (a Chinese indigenous breed). Our results of statistical analysis showed that the SNP had a highly significant association with the level of anti-CSFV antibody (At d 20; At d 35) in serum (p = 0.008; p = 0.0001). Investigation of expression and polymorphisms of the porcine FcRn gene will help us in further understanding the molecular basis of the antibody regulation pathway in the porcine immune response. All these results indicate that FcRn gene might be regarded as a molecular marker for genetic selection of anti-CSFV antibody level in pig disease resistance breeding programmes.
机译：新生儿Fc受体（FcRn）基因编码与单体免疫球蛋白G（IgG）的Fe区结合并负责IgG转运和稳定的受体。在此报告中，鉴定了8900 bp的猪FcRn基因组DNA结构，推定的FcRn蛋白包含356个氨基酸。猪FcRn氨基酸序列与其他物种的同源性的比对和系统发育分析显示出高度同一性。通过实时定量聚合酶链反应（Q-PCR）检测FcRn mRNA在组织中的表达，结果显示FcRn在十个分析组织中广泛表达。通过DNA测序分析证实了猪FcRn基因外显子6区域的一个单核苷酸多态性（SNP）（HQ026019：g.8526 C> T）。在包括大白猪，长白猪和松辽黑猪（中国本土品种）在内的三个猪群中，对与血清经典猪瘟病毒抗体（抗CSFV）浓度相关的SNP基因型进行了进一步分析。我们的统计分析结果表明，SNP与血清中抗CSFV抗体水平（第20天;第35天）高度相关（p = 0.008; p = 0.0001）。猪FcRn基因表达和多态性的研究将有助于我们进一步了解猪免疫应答中抗体调控途径的分子基础。所有这些结果表明，在猪抗病育种计划中，FcRn基因可能被视为抗CSFV抗体水平遗传选择的分子标记。

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