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首页> 外文期刊>Breeding science >Construction of a linkage map based on retrotransposon insertion polymorphisms in sweetpotato via high-throughput sequencing
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Construction of a linkage map based on retrotransposon insertion polymorphisms in sweetpotato via high-throughput sequencing

机译：基于高通量测序的甘薯逆转座子插入多态性连锁图谱的构建

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摘要

Sweetpotato (Ipomoea batatas L.) is an outcrossing hexaploid species with a large number of chromosomes (2n = 6x = 90). Although sweetpotato is one of the world's most important crops, genetic analysis of the species has been hindered by its genetic complexity combined with the lack of a whole genome sequence. In the present study, we constructed a genetic linkage map based on retrotransposon insertion polymorphisms using a mapping population derived from a cross between 'Purple Sweet Lord' (PSL) and '90IDN-47' cultivars. High-throughput sequencing and subsequent data analyses identified many Rtsp-1 retrotransposon insertion sites, and their allele dosages (simplex, duplex, triplex, or double-simplex) were determined based on segregation ratios in the mapping population. Using a pseudo-testcross strategy, 43 and 47 linkage groups were generated for PSL and 90IDN-47, respectively. Interestingly, most of these insertions (similar to 90%) were present in a simplex manner, indicating their utility for linkage map construction in polyploid species. Additionally, our approach led to savings of time and labor for genotyping. Although the number of markers herein was insufficient for map-based cloning, our trial analysis exhibited the utility of retrotransposon-based markers for linkage map construction in sweetpotato.
机译：甘薯（Ipomoea batatas L.）是具有大量染色体（2n = 6x = 90）的异六倍体物种。尽管甘薯是世界上最重要的农作物之一，但由于其遗传复杂性以及缺乏完整的基因组序列，对该物种的遗传分析产生了阻碍。在本研究中，我们使用“紫色甜王”（PSL）和“ 90IDN-47”品种之间杂交的作图种群，基于反转录转座子插入多态性构建了遗传连锁图谱。高通量测序和后续数据分析确定了许多Rtsp-1逆转录转座子插入位点，并根据作图群体中的分离比确定了它们的等位基因剂量（单重，双重，三重或双重）。使用伪testcross策略，分别为PSL和90IDN-47生成了43个和47个链接组。有趣的是，这些插入物中的大多数（约90％）以单工方式存在，表明它们可用于多倍体物种中的连锁图构建。此外，我们的方法还节省了基因分型的时间和劳动力。尽管此处的标记数量不足以进行基于图的克隆，但我们的试验分析显示，基于反转录转座子的标记可用于甘薯中连锁图的构建。

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