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首页> 外文期刊>Brain, Behavior, and Immunity >Alteration of serotonin transporter messenger RNA level in the peripheral blood mononuclear cells from simian/human immunodeficiency virus infected Chinese rhesus macaques (Macaca mulatta).
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Alteration of serotonin transporter messenger RNA level in the peripheral blood mononuclear cells from simian/human immunodeficiency virus infected Chinese rhesus macaques (Macaca mulatta).

机译：猴/人免疫缺陷病毒感染的中华猕猴（Macaca mulatta）外周血单核细胞中血清素转运蛋白信使RNA水平的变化。

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Serotonin transporter (SERT, 5-HTT) is a key element in the serotonergic system which is probably involved in the psychiatric disorders commonly observed in people living with HIV/AIDS. However, no information is available about the effects of HIV infection on SERT expression. In this study, a TaqMan real-time RT-PCR method was established, levels of SERT mRNA in the peripheral blood mononuclear cells (PBMCs) and various tissues from normal Chinese rhesus macaques, in PBMCs from 32 SHIV-sf162p4 infected rhesus macaques and from 8 rhesus macaques before and 7, 14, 21, 28 and 196 days after SHIV-sf162p4 infection, and in PBMCs before and after in vitro phytohemagglutinin (PHA) stimulation were examined. It was found that SERT mRNA was widely distributed in lymphoid tissues; the level of SERT mRNA was significantly reduced in PBMCs from SHIV infected rhesus macaques and in PBMCs stimulated with PHA. The most evident decrease (to about one-tenth) in SERT mRNA level was observed at day 7 after SHIV infection. Difference in PBMC SERT mRNA level between 5-HTTLPR genotypes was not statistically significant. These data indicated that, in addition to previously observed abnormality in serotonin metabolism, SERT expression might be affected in HIV/AIDS, which might be associated with depression and other psychiatric disorders in HIV/AIDS. Besides, this study provided a basis for quantitative analysis of SERT gene expression under effects of host and environmental factors, such as 5-HTTLPR genotypes, SERT targeting drugs or other infectious agents.
机译：5-羟色胺转运蛋白（SERT，5-HTT）是5-羟色胺能系统中的关键因素，可能与艾滋病毒/艾滋病患者常见的精神病有关。但是，没有有关HIV感染对SERT表达的影响的信息。在这项研究中，建立了TaqMan实时RT-PCR方法，检测了来自正常中华恒河猴的外周血单核细胞（PBMC）和各种组织，来自32株受SHIV-sf162p4感染的恒河猴的PBMC和来自正常恒河猴的PBMC中SERT mRNA的水平。检查了在SHIV-sf162p4感染之前和之后，7、14、21、28和196天的8只恒河猴，以及体外植物血凝素（PHA）刺激之前和之后的PBMC中。发现SERT mRNA广泛分布在淋巴组织中。 SHIV感染的恒河猴的PBMC和PHA刺激的PBMC中SERT mRNA的水平显着降低。在SHIV感染后第7天观察到SERT mRNA水平最明显的下降（约十分之一）。 5-HTTLPR基因型之间的PBMC SERT mRNA水平差异无统计学意义。这些数据表明，除了先前观察到的5-羟色胺代谢异常外，SERT表达可能在HIV / AIDS中受到影响，这可能与HIV / AIDS中的抑郁症和其他精神疾病有关。此外，本研究为在宿主和环境因素（例如5-HTTLPR基因型，靶向SERT的药物或其他感染因子）影响下SERT基因表达的定量分析提供了基础。

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