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首页> 外文期刊>Comparative Medicine >Constitutive Release of IFNy and IL2 from Peripheral Blood Mononuclear Cells of Rhesus Macaques (Macaca mulatto) Infected with Simian T-Lymphotropic Virus Type 1
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Constitutive Release of IFNy and IL2 from Peripheral Blood Mononuclear Cells of Rhesus Macaques (Macaca mulatto) Infected with Simian T-Lymphotropic Virus Type 1

机译：从猿猴T-淋病病毒感染的恒河猴（猕猴）的外周血单个核细胞中本构释放IFNγ和IL2。

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Simian T-cell lymphotropic viruses (STLV), the nonhuman primate counterparts of human T-cell lymphotropic viruses (HTLV), are endemic in many populations of African and Asian monkeys and apes. Although an etiologic link between STLV1 infection and lymphoproliferative disorders such as malignant lymphomas has been suggested in some nonhuman primate species, most STLV infections are inapparent, and infected animals remain clinically healthy. The retroviral transactivator, tax, is well known to increasetranscription of viral and cellular genes, resulting in altered cytokine profiles. This study compared the cytokine profiles of peripheral blood mononuclear cell (PBMC) cultures from 25 STLVl-seropositive rhesus macaques (Macaca mulatto) with those of age- and sex-matched seronegative controls. IFNy, TNFoc, IL10, and IL2 levels in unstimulated PBMC culture supernatants were measured at 24,48, and 72 h by using enzyme immunoassays. IFNgamma concentrations were found significantly higher in the supernatants of PBMC cultures of seropositive monkeys as compared with seronegative controls. In addition, although IL2 concentrations were not significantly elevated in the supernatants of PBMC cultures of all seropositive monkeys as compared with all seronegative controls, IL2 levels were increased in a subset of 5 pairs. Increased constitutive cytokine release occurred in the absence of spontaneous proliferation. The increased constitutive release of IFNgamma and IL2 suggests that STLV1 alters immune functions in infected but clinically healthy rhesus macaques and further characterizes STLV1 infection of rhesus macaques as a potential model for human HTLV1 infection.
机译：猿猴T细胞淋巴病毒（STLV）是人类T细胞淋巴病毒（HTLV）的非人类灵长类动物对应动物，在非洲和亚洲猴猴的许多种群中都很流行。尽管在某些非人类灵长类动物中已提出STLV1感染与淋巴增生性疾病（例如恶性淋巴瘤）之间的病因学联系，但大多数STLV感染是不明显的，并且被感染的动物在临床上仍然健康。众所周知，逆转录病毒反式激活因子可增加病毒和细胞基因的转录，从而导致细胞因子谱改变。这项研究比较了年龄和性别相匹配的血清阴性对照中来自25种STLV1血清阳性恒河猴（猕猴）的外周血单核细胞（PBMC）培养物的细胞因子谱。使用酶免疫法分别在24,48和72 h测量未刺激的PBMC培养上清液中的IFNγ，TNFα，IL10和IL2水平。发现与血清阴性对照相比，血清反应阳性猴子的PBMC培养上清中的IFNγ浓度明显更高。另外，尽管与所有血清阴性对照相比，所有血清反应阳性猴子的PBMC培养物的上清液中IL2浓度均未显着升高，但在5对中，IL2水平升高。在不存在自发增殖的情况下，本构细胞因子释放增加。 IFNgamma和IL2的组成型释放增加表明STLV1改变了已感染但临床上健康的恒河猴的免疫功能，并进一步将恒河猴的STLV1感染表征为人HTLV1感染的潜在模型。

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