• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Asian journal of drug metabolism and pharmacokinetics >Determination of Gliclazide in human plasma by RP-HPLC
【24h】

Determination of Gliclazide in human plasma by RP-HPLC

机译：RP-HPLC法测定人血浆中格列齐特

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Aim To establish a simple and sensitive high performance liquid chromatographic (HPLC) method involving ultraviolet detecting for the determination of Gliclazide in human plasma. Methods A diamonsil C18 column (200X4.6mm ID, 5um) was used as stationary phase and the mobile phase consisted of acetonitrile and water (49:51, v/v, pH2.8) at a flow-rate of 1.2 mL min~(-1). The Ultraviolet absorbance was monitored at 229nm wavelength. Temperature control was kept at 45 癈 for the column. Results The lower limit of quantitation achieved was 50ng-mL~(-1), and the standard curve was found to be linear in the concentration range of 50-l0000ng-mL~(-1). Gliclazide and internal standard were eluted at 8.5min and 4.8min respectively. Conclusion The method was very good and suitable for determination of Gliclazide in human plasma.
机译：目的建立一种简单，灵敏的高效液相色谱（HPLC）方法，该方法涉及紫外检测，用于测定人血浆中格列齐特。方法使用diamonsil C18色谱柱（200X4.6mm ID，5um）作为固定相，流动相由乙腈和水（49:51，v / v，pH2.8）组成，流速为1.2 mL min〜 （-1）。在229nm波长下监测紫外线吸收。将该柱的温度控制在45℃。结果定量下限为50ng-mL〜（-1），标准曲线在50-10000ng-mL〜（-1）浓度范围内呈线性关系。格列齐特和内标分别在8.5min和4.8min洗脱。结论该方法非常准确，适用于人血浆中格列齐特的测定。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号