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首页> 外文期刊>Australian Dental Journal >Effect of ginsenoside Rg1 on proliferation and differentiation of human dental pulp cells in vitro
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Effect of ginsenoside Rg1 on proliferation and differentiation of human dental pulp cells in vitro

机译：人参皂苷Rg1对人牙髓细胞体外增殖和分化的影响

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摘要

Background: Human dental pulp cells (hDPCs) have the potency to proliferate and differentiate into odontoblasts and play an important role in dentine formation and reparation. The aim of the present study is to evaluate the effects of ginsenoside Rg1 on the proliferation and differentiation of hDPCs. Methods: hDPCs were incubated with different concentrations of ginsenoside Rg1 (0.1, 0.5, 2.5, 5, 10 and 20 μmol/L). The effects of ginsenoside Rg1 on the proliferative ability of hDPCs were evaluated by a fibroblast colony forming test, MTT assay and flow cytometry for cell cycle. The control group, osteogenic induction group, ginsenoside Rg1 (5 μmol/L) group and combination group were designed, and alkaline phosphatase (ALP) activity and FQ-PCR for gene expressions of dentine sialophosphoprotein (DSPP) and dentine matrix protein 1 (DMP1) were performed to evaluate the differentiation of hDPCs. Results: The proliferative ability of hDPCs in ginsenoside Rg1 was significantly enhanced (p < 0.05), especially in the ginsenoside Rg1 (5 μmol/L) group. ALP activity and gene expressions of DSPP and DMP1 were increased in the induction group, ginsenoside Rg1 group, and their combination group compared with the control group (p < 0.05). Conclusions: The results indicate that ginsenoside Rg1 promotes the proliferation and differentiation of hDPCs.
机译：背景：人类牙髓细胞（hDPC）具有增殖能力并分化为成牙本质细胞，并在牙本质的形成和修复中发挥重要作用。本研究的目的是评估人参皂苷Rg1对hDPCs增殖和分化的影响。方法：将hDPC与不同浓度的人参皂苷Rg1（0.1、0.5、2.5、5、10和20μmol/ L）孵育。人参皂甙Rg1对hDPCs增殖能力的影响通过成纤维细胞集落形成试验，MTT测定和流式细胞仪评估细胞周期。设计对照组，成骨诱导组，人参皂苷Rg1（5μmol/ L）组和联合组，并用碱性磷酸酶（ALP）活性和FQ-PCR检测牙本质唾液磷蛋白（DSPP）和牙本质基质蛋白1（DMP1）的基因表达。进行了评估hDPC的分化。结果：hDPCs在人参皂苷Rg1中的增殖能力显着增强（p <0.05），特别是在人参皂苷Rg1（5μmol/ L）组中。与对照组相比，诱导组，人参皂苷Rg1组及其联合组的ALP活性和DSPP和DMP1基因表达均升高（p <0.05）。结论：结果表明人参皂苷Rg1促进hDPCs的增殖和分化。

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