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首页> 外文期刊>Australasian Plant Pathology >RFLP analysis of rDNA-ITS regions of native non-pathogenic Fusarium oxysporum isolates and their field evaluation for the suppression of Fusarium wilt disease of banana
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RFLP analysis of rDNA-ITS regions of native non-pathogenic Fusarium oxysporum isolates and their field evaluation for the suppression of Fusarium wilt disease of banana

机译：天然非致病性尖孢镰刀菌菌株rDNA-ITS区域的RFLP分析及对香蕉枯萎病的抑制作用现场评估

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Fusarium wilt caused by Fusarium oxysporum f. sp. cubense is the most devastating disease of banana affecting commercial cultivars grown worldwide. An attempt has been made to identify antagonistic, non-pathogenic Fusarium oxysporum (npFo) isolatesfrom banana soil. A total of 200 rhizosphere soil samples were collected from different commercial cultivars, as well as wild bananas. Forty Fusarium isolates were recovered, of which 33 were identified as Fo based on mycelial and spore characters. The identity of Fo isolates was confirmed by specific primers FOF1 and FOR1 in PCR reactions. The 33 isolates were confirmed as non-pathogenic following inoculation studies. Molecular characterisation of the npFo isolates was determined by ribosomal internally transcribed spacer (ITS) restriction fragment length polymorphism. Pathogenic isolates were also included for comparison. Among the npFo isolates, 14 ITS haplotypes were observed of which groups 6 and 10 were major groups consisting of 6 and 5 npFo isolates, respectively.The in vitro evaluation of these npFo isolates by dual culture plate and spore germination technique against the Fusarium wilt pathogen revealed the most inhibiting isolates were Ro-3 and Ra-1. Under pot culture studies, these npFo isolates were evaluated by application: (i) at planting; (ii) at planting + 2 months after planting; and (iii) at planting + 2 months after planting + 4 months after planting, in both tissue-cultured as well as sucker-derived plants of the cultivar Rasthali. The Fusarium wilt severity score observed after 6 months demonstrated that the application of Ro-3 three times resulted in the reduction of Fusarium wilt severity by up to 89% as well as increasing the plant growth parameters significantly when compared with the pathogen-inoculated control plants. Field application of these npFo isolates also reduced Fusarium wilt disease by 80%. The results of this study suggest that npFo strain Ro-3 could be used for the management of Fusarium wilt disease of banana to sustain banana production.
机译：尖孢镰刀菌引起的枯萎病f。 sp。立方体病是影响世界范围内种植的商业品种的最具破坏性的香蕉病。已尝试鉴定香蕉土壤中的拮抗性，非致病性的尖孢镰刀菌（npFo）分离株。总共从不同的商业品种以及野生香蕉中收集了200个根际土壤样品。回收了40种镰刀菌分离物，根据菌丝和孢子特征鉴定出33种为Fo。通过PCR反应中的特异性引物FOF1和FOR1确认Fo分离物的身份。接种研究后确认了33株分离物为非病原体。 npFo分离物的分子表征是通过核糖体内部转录间隔区（ITS）限制性片段长度多态性来确定的。还包括致病性分离株用于比较。在npFo菌株中，观察到14种ITS单倍型，其中第6组和第10组分别是由6和5个npFo菌株组成的主要组。双重培养板和孢子萌发技术对这些npFo菌株的体外评价揭示了最具抑制作用的分离株是Ro-3和Ra-1。在盆栽研究中，通过应用评估了这些npFo分离株：（i）在种植时; （ii）播种时+播种后2个月; （iii）在栽培中+在栽培后2个月+在栽培后4个月，无论是组织培养的还是吸盘栽培的栽培品种Rasthali的植物。 6个月后观察到的枯萎病严重程度评分表明，与接种病原体的对照植物相比，施用3次Ro-3可降低高达89％的枯萎病严重程度，并显着提高植物生长参数。这些npFo分离物的现场应用还减少了80％的枯萎病。这项研究的结果表明，npFo Ro-3菌株可用于处理香蕉枯萎病，以维持香蕉的产量。

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