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首页> 外文期刊>Assiut Veterinary Medical Journal >Comparative study on the activity of 5'-nucleotidase 5-NT and gamma-glutamyltransferase (GGT) in some tissues of donkeys, calves and goats (the effect of freezing and thawing).
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Comparative study on the activity of 5'-nucleotidase 5-NT and gamma-glutamyltransferase (GGT) in some tissues of donkeys, calves and goats (the effect of freezing and thawing).

机译：驴，犊牛和山羊某些组织中5'-核苷酸酶5-NT和γ-谷氨酰胺转移酶（GGT）活性的比较研究（冷冻和解冻作用）。

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摘要

5'-nucleotidase (5'-NT) and gamma-glutamyltransferase (GGT) activity was measured in the tissues of four 2- to 6-year-old donkeys, four 6- to 9-month-old castrated male goats and four 6- to 9-month-old male calves. It was found that the lungs is the greatest source of 5'-NT in donkeys, whereas in goats, the liver is the primary source of the enzyme. Generally, the values of 5'-NT activities in the tissues of calves and goats were higher than those of donkeys. GGT was mainly located in the kidneys, pancreas and liver of all the species studied. A 20-25% loss in activity of GGT occurred after thawing and freezing of tissues for 2 weeks. While a loss of more than 50% in the activity of the enzyme occurred in within four weeks. The fall in the activity of 5'-NT in tissues was not consistent in all tissues during the first two weeks, but after four weeks, the 50% loss of activity was observed..
机译：在四只2至6岁大的驴，四只6至9个月大month割的公山羊和四只6只大公羊的组织中测量了5'-核苷酸酶（5'-NT）和γ-谷氨酰转移酶（GGT）活性。 -9个月大的雄性犊牛。已发现，肺是驴中5'-NT的最大来源，而在山羊中，肝脏是酶的主要来源。通常，犊牛和山羊组织中5'-NT活性的值高于驴。 GGT主要位于所有研究物种的肾脏，胰腺和肝脏中。解冻和冷冻组织2周后，GGT活性降低了20-25％。而在四周之内发生的酶活性损失超过50％。在最初的两周内，组织中5'-NT活性的下降并非一致，但在四周后，观察到50％的活性下降。

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