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首页> 外文期刊>Australasian Plant Pathology >Genetic variability amongst isolates of Australian grapevine phytoplasmas
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Genetic variability amongst isolates of Australian grapevine phytoplasmas

机译:澳大利亚葡萄植原体分离株之间的遗传变异

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摘要

Heteroduplex mobility assay (HMA) was used to determine if genetic variability existed amongst isolates of Australian grapevine yellows (AGY) and the Buckland Valley grapevine yellows (BVGY) phytoplasmas. Genetic variability was detected in two isolates of AGY phytoplasma from the same grapevine when they were compared with an AGY phytoplasma standard using HMA of the elongation factor Tu (tuf) gene. HMA of the tuf gene also showed that an isolate of papaya dieback (PDB) phytoplasma, which is closely related to AGY phytoplasma, was different from the AGY phytoplasma standard. The two AGY variant phytoplasmas and PDB phytoplasma were indistinguishable from each other when they were compared using HMA of the tuf gene. No variability was observed amongst isolates of BVGY phytoplasma using HMA of the tuf gene.
机译:异源双链迁移率分析(HMA)用于确定澳大利亚葡萄黄(AGY)和巴克兰谷葡萄黄(BVGY)植原体分离株之间是否存在遗传变异。使用延伸因子Tu(tuf)基因的HMA与来自同一葡萄树的两种AGY植物原质分离株进行遗传变异分析,并将它们与AGY植物原质标准进行比较。 tuf基因的HMA还显示,与AGY植物原质密切相关的木瓜枯死(PDB)植物原质分离株不同于AGY植物原质标准。当使用tuf基因的HMA进行比较时,这两个AGY变异植物质体和PDB植物质体是无法区分的。使用tuf基因的HMA,在BVGY植物质体分离株中未观察到变异性。

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