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Detection of Listeria monocytogenes from food samples by PCR after IMS-plating

机译：IMS铺板后通过PCR检测食品样品中的李斯特菌

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摘要

For rapid, accurate and sensitive detection of Listeria monocytogenes in food samples, colonies developed on the selective agar (Oxford agar) after immunomagnetic separation (IMS) were subjected to polymerase chain reaction (PCR) assay with the prf A1-2 primer pair. The proposed assay system was shown experimentally to be capable of specifically detecting the bacteria from food samples contaminated at more than 10(2) cfu/g. However, the enrichment culture after a short period of 16 h with the appropriate selective broth was needed before IMS-plating, because the bacterial contents in most actual food were as low as less than 10(2) cfu/g. However, even if the enrichment cultivation was employed before IMS, L. monocytogenes was detected within 3 days.
机译：为了快速，准确和灵敏地检测食品样品中的单核细胞增生李斯特菌，对在免疫磁分离（IMS）之后在选择性琼脂（Oxford琼脂）上形成的菌落进行prf A1-2引物对聚合酶链反应（PCR）分析。实验表明，所提出的测定系统能够特异性地检测出食品样本中细菌的细菌含量超过10（2）cfu / g。但是，在IMS铺板之前，需要在短短16 h后用适当的选择性肉汤进行富集培养，因为大多数实际食品中的细菌含量低至低于10（2）cfu / g。但是，即使在IMS之前进行了富集培养，在3天内仍会检测到单核细胞增生李斯特菌。

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