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Metal-mediated control of the activity of Bacillus subtilis superoxide dismutase

机译：金属介导的枯草芽孢杆菌超氧化物歧化酶活性的控制

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摘要

A Bacillus subtilis sodA recombinant plasmid, pMW-sod, was introduced into a Sod-deficient strain E. coil IM303 (sodA, sodB). The activity of superoxide dismutase in the crude extract of E. coil IM303 bearing pMW-sod was markedly increased by the addition of an inducer, isopropyl-#beta#-D-thiogalactopyranoside, in Mn~(2+)-supplemented medium; in contrast, it was kept at the basal level in Fe~(2+)-supplemented medium. However, the specific activity of B. subtilis SodA purified from this strain was extremely low, compared with that from B. subtilis. An atomic absorption analysis revealed that a relatively high content of Fe~(2+) was bound to this purified B. subtilis SodA. We further demonstrated that this purified B. subtilis Soda was activated by incubation with manganese salt. These results suggest that 8. subtilis Soda was posttranslationally regulated by the manganese in vivo and in vitro.
机译：将枯草芽孢杆菌的sodA重组质粒pMW-sod引入到Sod缺陷菌株大肠杆菌IM303（sodA，sodB）中。在添加了Mn〜（2+）的培养基中加入诱导剂异丙基-β-β-D-硫代半乳糖吡喃糖苷，可显着提高带有pMW-sod的IM.303 IM303粗提物粗提物中超氧化物歧化酶的活性。相比之下，在补充有Fe〜（2+）的培养基中它保持在基础水平。但是，与枯草芽孢杆菌相比，从该菌株纯化的枯草芽孢杆菌SodA的比活性极低。原子吸收分析表明，相对较高的Fe〜（2+）含量与该纯化的枯草芽孢杆菌SodA结合。我们进一步证明了该纯化的枯草芽孢杆菌苏打是通过与锰盐孵育而被激活的。这些结果表明，枯草杆菌苏打在体内和体外均受锰的翻译后调控。

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