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Comparison of Bacillus subtilis strains from a food poisoning outbreak and other sources analyzed by pulsed-field gel electrophoresis

机译：食物中毒暴发和其他来源的枯草芽孢杆菌菌株的比较，通过脉冲场凝胶电泳进行分析

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摘要

Ten strains of Bacillus subtilis from different sources were genotyped by pulsed-field gel electrophoresis (PFGE) after separate digestion with Acc II, BamH I or Hind III. Among the ten strains tested, five strains originated from patients and the food source incriminated in a B. subtil/s food poisoning outbreak, four from foods and soil epidemiologically unrelated to the outbreak, and one was B. subtilis PCI 219. Although five strains from the outbreak displayed the same PFGE pattern after digestion of chromosomal DNA by three restriction endonucleases, the other strains showed distinctly different patterns from each other. Thus, the PFGE analysis was suggested to provide an useful tool for the epidemiological survey on B. subtilis food poisoning outbreaks.
机译：在用Acc II，BamH I或Hind III分别消化后，通过脉冲场凝胶电泳（PFGE）对十个来自不同来源的枯草芽孢杆菌菌株进行基因分型。在所测试的十个菌株中，有五株来自患者和食物来源，是由枯草芽孢杆菌引起的食物中毒暴发，有四株来自与该病的流行病学无关的食物和土壤，还有一株是枯草芽孢杆菌PCI219。尽管有五株从暴发中分离出的三株限制性内切酶消化了染色体DNA后，它们显示出相同的PFGE模式，其他菌株则表现出明显不同的模式。因此，建议进行PFGE分析为枯草芽孢杆菌食物中毒暴发的流行病学调查提供有用的工具。

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