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Dichloromethane-degrading properties of bacteria isolated from environmental water

机译：从环境水中分离出的细菌对二氯甲烷的降解特性

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摘要

Degradation of dichloromethane (DCM) by two environmental isolates, Flavimonas sp. strain P3310 and Chryseobacterium sp. strain G31, were studied. The ability of the strains was raised to degrade 3,000 mg/l of DCM by acclimatization, although the original isolates could degrade less than 500 mg/l. The first step in the degradation process was dechlorination, and the liberated chloride ions caused the reduction of pH and the bacterial growth; the addition of phosphate salts, however, restored the growth and the degrading ability of the culture by increasing the buffer capacity. The DCM-degrading activity was also detected in the cell-free extract and the culture-supernatant. These results suggest that the isolates or their products are possible candidates for bioremediation to eliminate DCM pollution.
机译：两种环境分离物Flavimonas sp。降解二氯甲烷（DCM）。 P3310菌株和Chryseobacterium sp。研究了菌株G31。尽管原始分离株的降解能力低于500 mg / l，但通过驯化提高了菌株降解3,000 mg / l DCM的能力。降解过程的第一步是脱氯，释放的氯离子导致pH降低和细菌生长。然而，通过增加缓冲容量，添加磷酸盐可以恢复培养物的生长和降解能力。在无细胞提取物中和培养上清液中也检测到DCM降解活性。这些结果表明，分离物或其产物可能是进行生物修复以消除DCM污染的候选物。

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