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首页> 外文期刊>Biocontrol Science >Increase in the intracellular hydrogen peroxide concentration in Escherichia coli cells treated with ethanol and its relation to cell death
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Increase in the intracellular hydrogen peroxide concentration in Escherichia coli cells treated with ethanol and its relation to cell death

机译：乙醇处理的大肠杆菌细胞中细胞内过氧化氢浓度的增加及其与细胞死亡的关系

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摘要

When Escherichia coli cells were treated with 20-40 percent (v/v) ethanol solution, there was an increase in intracellular concentration of hydrogen peroxide accompanied by a decrease in the survival rate of the cells. Several mutants with higher catalase activity were more resistant and some other mutants with lower catalase activity were more sensitive to ethanol treatment than the parent strain K12. The concentration of hydrogen peroxide in the ethanol-treated E. coli K12 cells was 20 to 160 times higher than that in the untreated cells. The catalase-gene kat E', kat G, and kat E cloned strains, having higher catalase activity, were more ethanol-resistant than E. coli K12, recipient strain UM1 and UM1 charomid 9-28. For the bactericidal mechanism of ethanol, it is suggested that ethanol treatment might cause some damage to the cellular aerobic respiratory system and as a result, hydrogen peroxide may be generated to result in cell death.
机译：用20-40％（v / v）乙醇溶液处理大肠杆菌细胞时，细胞内过氧化氢浓度增加，同时细胞存活率降低。与亲本菌株K12相比，一些具有较高过氧化氢酶活性的突变体具有更高的抗性，而另一些具有较低过氧化氢酶活性的突变体对乙醇处理更敏感。乙醇处理过的大肠杆菌K12细胞中过氧化氢的浓度比未处理过的细胞高20至160倍。具有较高过氧化氢酶活性的过氧化氢酶基因kat E'，kat G和kat E克隆菌株比大肠杆菌K12，受体菌株UM1和UM1 charomid 9-28具有更高的乙醇抗性。关于乙醇的杀菌机理，建议乙醇处理可能会对细胞的需氧呼吸系统造成一定的损害，结果，可能会产生过氧化氢导致细胞死亡。

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