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首页> 外文期刊>Biodegradation >Biodegradation of kraft-lignin by Bacillus sp. isolated from sludge of pulp and paper mill.
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Biodegradation of kraft-lignin by Bacillus sp. isolated from sludge of pulp and paper mill.

机译：芽孢杆菌属物种对牛皮纸木质素的生物降解作用。从纸浆和造纸厂的污泥中分离出来。

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Eight bacterial strains were isolated on kraft lignin (KL) containing mineral salt medium (L-MSM) agar with glucose and peptone from the sludge of pulp and paper mill. Out of these, ITRC-S8 was selected for KL degradation, because of its fast growth at highest tested KL concentration and use of various lignin-related low molecular weight aromatic compounds (LMWACs) as sole source of carbon and energy. The bacterium was identified by biochemical tests as Gram-positive, rod-shaped and non-motile. Subsequent 16S rRNA gene sequencing showed 95% base sequence homology and it was identified as Bacillus sp. In batch experiments, a decrease in pH was observed initially followed by an increase till it reached an alkaline pH, which did not alter the culture growth significantly. The bacterium reduced the colour and KL content of 500 mg l(-1 )KL in MSM, in the presence of glucose and peptone, at pH 7.6, temperature 30 degrees C, agitation of 120 rpm and 6 days of incubation by 65 and 37% respectively. Significantreduction in colour and KL content in subsequent incubations is indicative of a co-metabolism mechanism, possibly due to initial utilization of added co-substrates for energy followed by utilization of KL as a co-metabolic. The degradation of KL by bacterium was confirmed by GC-MS analysis indicating formation of several LMWACs such as t-cinnamic acid, 3, 4, 5-trimethoxy benzaldehyde and ferulic acid as degradation products, which were not present in the control (uninoculated) sample. This favours the idea of biochemical modification of the KL polymer to a single monomer unit.
机译：从纸浆和造纸厂的污泥中，在含有矿物盐培养基（L-MSM）琼脂的硫酸盐木质素（KL）琼脂上分离了8个细菌菌株。其中，选择ITRC-S8进行KL降解是因为它在最高的KL浓度下能快速生长，并且使用各种木质素相关的低分子量芳族化合物（LMWAC）作为唯一的碳和能源。该细菌通过生化试验鉴定为革兰氏阳性，杆状和不活动。随后的16S rRNA基因测序显示95％的碱基序列同源性，被鉴定为芽孢杆菌。在分批实验中，最初观察到pH降低，然后升高直至达到碱性pH，这不会显着改变培养物的生长。在葡萄糖和蛋白ept存在下，pH 7.6，温度30摄氏度，搅拌120 rpm和65和37孵育6天的情况下，细菌降低了MSM中500 mg l（-1）KL的颜色和KL含量％ 分别。在随后的孵育中，颜色和KL含量的显着降低表明存在共代谢机制，这可能是由于最初利用添加的共底物获得能量，然后利用KL作为共代谢物。 GC-MS分析证实了细菌对KL的降解，表明形成了一些LMWAC，例如叔肉桂酸，3、4、5-三甲氧基苯甲醛和阿魏酸作为降解产物，它们在对照中不存在（未接种）样品。这有利于将KL聚合物生化修饰为单个单体单元的想法。

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