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Effect of high pressure gaseous and supercritical Carbon dioxide treatments on bacterial spores

机译：高压气态和超临界二氧化碳处理对细菌孢子的影响

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摘要

Effects of high pressure gaseous carbon dioxide treatment (35 deg C, 6.5 MPa) (HGCT) and supercritical carbon dioxide treatment (35 deg C, 30 MPa) (HSCT), followed by heat treatment at 70 deg C (criterion for germination), on Bacillus coagulans andB. licheniformis spores were investigated. In both strains, HSCT was more effective in inactivating the bacterial spores than HGCT. Approximately 10 percent of the spores of B. coagulans were inactivated, and 40 percent of the B. coagulans spores germinated with HGCT for 120 min. Approximately 90 percent of the spores of B, coagulans were inactivated and germinated with HSCT for 120 min. Approximately 20 percent of the spores of B. licheniformis were inactivated, and 70 percent of the B. licheniformis spores germinated with HGCT for 120 min. Approximately 80 percent of the spores of B. licheniformis were inactivated, and 90 percent of the B. licheniformis spores germinated with HSCT at 120 min. These results suggest that the spores germinate more effectively with HSCT than HGCT, and germinated spores are more sensitive to HSCT than HGCT.
机译：高压气态二氧化碳处理（35℃，6.5 MPa）（HGCT）和超临界二氧化碳处理（35℃，30 MPa）（HSCT），然后在70℃进行热处理（发芽标准）的影响，对凝结芽胞杆菌和B.调查地衣形孢子。在两种菌株中，HSCT均比HGCT更有效地灭活细菌孢子。大约有10％的凝结芽孢杆菌孢子被灭活，而40％的凝结芽孢杆菌孢子通过HGCT萌发了120分钟。约90％的B凝集素孢子被灭活并用HSCT萌发120分钟。大约20％的地衣芽孢杆菌孢子被灭活，并且70％的地衣芽孢杆菌孢子通过HGCT萌发了120分钟。大约80％的地衣芽孢杆菌孢子被灭活，并且90％的地衣芽孢杆菌孢子在120分钟时被HSCT萌发。这些结果表明，与HGCT相比，HSCT能更有效地发芽孢子，而与HGCT相比，发芽的孢子对HSCT更敏感。

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