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Correlation between the bacterioclastic action of a bis-quaternary ammonium compound and outer membrane proteins

机译：双季铵盐化合物的细菌破灭作用与外膜蛋白的相关性

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Bis-quaternary ammonium compounds (bis-QACs) have the ability to cause a rapid and abundant leakage of the turbid materials from cells, and such a bacterioclastic ability leads to a potent bactericidal activity. In order to clarify the detailed mechanism of the bactericidal action of bis-QACs, the correlation between the bacterioclastic action of 4,4'-(1,6-hexamethylenedithio)bis(1-octylpyridinium bromide) (4DTBP-6,8) and the leakage of outer membrane pore protein E (OmpE) was investigated. Using the antiserum against a fusion protein consisting of GST and the OmpE protein of Escherichia coli encoded by the ompE gene, it was seen that the leakage of OmpE from E. coli cells was caused by treatment with low concentrations (much lower than the critical vesiculation concentration) of 4DTBP-6,8. Furthermore, it was confirmed that 4DTBP-6,8 caused an increase in the turbidity of the cell suspension of Klebsiella pneumoniae, Salmonella typhimurium and Serratia marcescences, and led to the leakage of several proteins which have a high percentage of homology with OmpE of E. coll. By immunoelectron microscopy investigation, it was revealed that the vesiculation from E. coli treated with 4DTBP-6,8 contains OmpE. In addition, the bacteriolytic action of 4DTBP-6,8 was investigated. The results suggested that the lysis of cells by bis-QACs was not an enzymatic action such as that by autolysin but a physical bacterioclastic action. Judging from these results, it is suggested that the leakage of OmpE is one of the major bacterioclastic actions of bis-QACs, and deals the bacterial cells a fatal blow.
机译：双季铵化合物（bis-QAC）具有引起浑浊物质从细胞中快速大量泄漏的能力，并且这种细菌破坏能力导致有效的杀菌活性。为了阐明bis-QAC杀菌作用的详细机理，4,4'-（1,6-六亚甲基二硫代）双（1-辛基溴化吡啶鎓）（4DTBP-6,8）和研究了外膜孔蛋白E（OmpE）的泄漏情况。使用针对由GST和由ompE基因编码的大肠杆菌的OmpE蛋白组成的融合蛋白的抗血清，可以发现OmpE从大肠杆菌细胞中的渗漏是由低浓度处理引起的（远低于临界囊泡浓度）4DTBP-6,8。此外，已证实4DTBP-6，8导致肺炎克雷伯菌，鼠伤寒沙门氏菌和沙雷氏菌的淡色细胞悬浮液的浊度增加，并导致与E的OmpE具有高度同源性的几种蛋白质的泄漏。科尔。通过免疫电子显微镜研究，发现用4DTBP-6，8处理的大肠杆菌囊泡中含有OmpE。此外，还研究了4DTBP-6,8的杀菌作用。结果表明，bis-QACs对细胞的裂解不是像自溶素那样的酶促作用，而是物理的细菌破灭作用。从这些结果判断，表明OmpE的泄漏是bis-QAC的主要细菌破灭作用之一，并给细菌细胞带来致命的打击。

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