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Morphological significance of cladosporium contaminants on materials and utensils in contact with food

机译：与食物接触的材料和器皿上的担子孢子污染物的形态学意义

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Cladosporium contaminants on materials and utensils that come into contact with food were morphologically investigated. The most common contaminants, C. cladosporioides and C. sphaerospermum, were detected on the samples. The morphological changes of the Cladosporium species were investigated by using stereoscopic, optical light, fluorescent, and scanning electron microscopes. Microscopically the Cladosporium contaminants were observed as aggregated dark brown spots, strongly pigmented, irregularly swollen, and in long chains. Using fluorescent microscopy, the Cladosporium mycelia were clearly stained with fluorescein diacetate as viable cells, but the old cells were mostly non-viable, as shown by staining with propidium iodide. The dynamics of the morphological changes showed that the penetrating mycelia were closely attached to the surface of the materials and utensils under investigation. These results provide information about the significance of Cladosporium contamination on materials and utensils in contact with food and may contribute to the control of fungal contamination.
机译：形态学研究了与食物接触的材料和器皿上的Cladosporium污染物。在样品上检测到最常见的污染物，克氏梭状芽胞杆菌和球孢梭菌。通过使用立体，光学，荧光和扫描电子显微镜研究了枝孢属的形态变化。显微镜下观察到的Cladosporium污染物为聚集的黑褐色斑点，色素沉着，不规则溶胀且呈长链状。使用荧光显微镜，克拉多孢菌丝体被二乙酸荧光素清楚地染色为活细胞，但旧细胞大部分不活，如碘化丙啶染色所示。形态变化的动力学表明，穿透的菌丝体紧密附着在所研究的材料和器皿的表面。这些结果提供了有关Cladosporium污染在与食物接触的材料和器具上的重要性的信息，并且可能有助于控制真菌污染。

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