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ELISA detection of Kudoa septempunctata in raw Paralichthys olivaceus (olive flounder) using a chicken anti-Kudoa antiserum.

机译:使用鸡肉抗-Kudoa抗血清的ELISA检测未加工的Paralichthys olivaceus(橄榄比目鱼)中的Kudoa septempunctata。

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摘要

Kudoa septempunctata is the causative agent of a foodborne disease associated with the consumption of raw Paralichthys olivaceus (olive flounder). Chickens were used to establish specific antibodies against K. septempunctata spores. A specific antiserum, CS#3, raised against sonicated spores, also recognized intact spores. The CS#3 antiserum showed high titers for sonicated and intact K. septempunctata spores and was suitable for both ELISA and immunohistochemical staining. Using homogenated raw olive flounder meat, the ELISA system detected more than 5.0x105 spores in 1 g of tissue, which was consistent with the number determined by microscopic examination. The preparation of rapid detection kits for K. septempunctata spores in P. olivaceus muscle tissue using immunochromatography with CS#3 antiserum should be useful for preventing the foodborne disease in the field.
机译:Kudoa septempunctata是食用与食用原始Paralichthys olivaceus(橄榄比目鱼)有关的食源性疾病的病原体。鸡被用来建立针对K. septempunctata孢子的特异性抗体。针对超声孢子产生的特定抗血清CS#3也可以识别完整的孢子。 CS#3抗血清对超声处理和完整的K. septempunctata孢子显示出高滴度,并且适用于ELISA和免疫组化染色。 ELISA系统使用均质的生比目鱼肉,在1 g组织中检测到超过5.0x10 5 孢子,与通过显微镜检查确定的数量一致。使用带有CS#3抗血清的免疫色谱法制备油橄榄假单胞菌孢子菌孢子快速检测试剂盒,对于预防该领域的食源性疾病应该是有用的。

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