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首页> 外文期刊>Biocontrol Science >Characterization of the Bacterial Community in the Sediment of a Brackish Lake with Oyster Aquaculture
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Characterization of the Bacterial Community in the Sediment of a Brackish Lake with Oyster Aquaculture

机译：用牡蛎养殖对咸淡湖沉积物中的细菌群落进行表征

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摘要

The physicochemical properties and bacterial community in sediments of Lake Shiraishi, a lake with brackish water, were characterized to elucidate the influence of oyster farming and seawater and freshwater inflow. Physicochemical analyses suggested the marine origin of the sediment at the mouth of the lake, while higher organic matter load and the resultant anaerobic, reductive condition of the sediments of the inner part were observed. The bacterial community in the sediments reflects these sediment environments: the bacterial community in the vicinities of oyster farms included sulfate-reducing bacteria (SRB), although sulfur-oxidizing bacteria (SOB) were found at all the sampling sites. In addition, similarity of the band profiles obtained with 16S ribosomal RNA gene (16S rDNA)-denaturing gradient gel electrophoresis (DGGE) decreased in proportion to the distance from the mouth of the lake to the oyster farms in the inner part. This study was able to characterize the microbial community shift in brackish lake sediments with an oyster aquaculture system through the molecular fingerprinting technique, DGGE, in relation to their physicochemical characteristics.
机译：白盐湖（咸水湖）的沉积物的理化特性和细菌群落的特征是阐明牡蛎养殖以及海水和淡水流入的影响。理化分析表明，沉积物的海洋起源在湖口，同时观察到较高的有机质负荷和内部沉积物的厌氧还原条件。沉积物中的细菌群落反映了这些沉积物环境：牡蛎养殖场附近的细菌群落包括硫酸盐还原菌（SRB），尽管在所有采样点都发现了硫氧化菌（SOB）。此外，用16S核糖体RNA基因（16S rDNA）变性梯度凝胶电泳（DGGE）获得的条带图谱的相似性随从湖口到内部牡蛎养殖场的距离成比例降低。这项研究通过分子指纹技术DGGE表征了牡蛎养殖系统中咸淡湖沉积物中微生物群落的变化及其理化特征。

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