• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biomedical Engineering: Applications, Basis and Communications >EVALUATION OF ANTI-CANCER ACTIVITY OF SURVIVIN siRNA DELIVERED BY FOLATE RECEPTOR-TARGETED POLYETHYLENE-GLYCOL LIPOSOMES IN K562-BEARING XENOGRAFT MICE
【24h】

EVALUATION OF ANTI-CANCER ACTIVITY OF SURVIVIN siRNA DELIVERED BY FOLATE RECEPTOR-TARGETED POLYETHYLENE-GLYCOL LIPOSOMES IN K562-BEARING XENOGRAFT MICE

机译：叶酸受体靶向的聚乙烯乙二醇脂质体在含K562异种移植物小鼠体内传递的Survivin siRNA的抗癌活性评估

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Objective: To investigate anti-cancer activity of the novel survivin siRNA Folate Receptor (FR)-targeted Polyethylene-Glycol Liposomes (PEG) liposomes in K562-bearing nude mice. Methods: The leukemia cell line K562 xenograft model was established in balb/c nuu mice, and survivin siRNA FR-targeted PEG liposomes was administrated by intraperitoneal (i.p.). The same volume of liposomes was administrated as placebo control. The real time PCR and western blotting were used to examine the knocking down effect. The tumor weight and size in nude mice was measured to evaluate the inhibitory effect in vivo. Results: The expression ratio of survivin mRNA in siRNA group was 0.35 ± 0.1 (survivin/GAPDH) vs. 1.85 ± 0.65 in control group with significant difference (p < 0.05), as well as protein level by western blotting analysis (p < 0.05). The results also showed the novel survivin siRNA liposomes could inhibit the growth of K562 in xenograft model. Conclusion: This novel survivin siRNA FR-targeted PEG liposome delivery system may be a potential gene therapy for the treatment of leukemia.
机译：目的：研究靶向survivin siRNA叶酸受体（FR）的新型聚乙二醇脂质体（PEG）脂质体在荷K562裸鼠中的抗癌活性。方法：在balb / c nu / nu小鼠中建立白血病细胞系K562异种移植模型，并通过腹膜内（i.p.）施用针对survivin siRNA FR的PEG脂质体。给予相同体积的脂质体作为安慰剂对照。实时PCR和蛋白质印迹用于检查敲除效果。测量裸鼠中的肿瘤重量和大小以评估其体内抑制作用。结果：siRNA组中survivin mRNA的表达率为0.35±0.1（survivin / GAPDH），而对照组为1.85±0.65，差异有统计学意义（p <0.05），蛋白质印迹法检测的蛋白水平（p <0.05） ）。结果还表明，新型survivin siRNA脂质体可以抑制异种移植模型中K562的生长。结论：靶向survivin siRNA FR的新型PEG脂质体递送系统可能是治疗白血病的潜在基因疗法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号