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首页> 外文期刊>Biomedical Engineering: Applications, Basis and Communications >SOI SUBSTRATE-BASED MICROFLUIDIC CHIP FOR CELL ELECTROFUSION
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SOI SUBSTRATE-BASED MICROFLUIDIC CHIP FOR CELL ELECTROFUSION

机译：用于细胞电融合的基于SOI基质的微流控芯片

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摘要

A microelectrode array-based cell electrofusion chip was fabricated by using the MEMS technique. Because of the short distance between two counter microelectrodes, the working voltage on this chip was only 1/100-1/20 as that in the traditional cell electrofusion method. Simulation method was used to analyze the on-chip electric field distribution and optimize the structure of the microelectrodes. The results showed the length and width of the microelectrode, and the distance between two microelectrodes in the horizontal and vertical direction would impact the strength and distribution of the electric field. Thus, optimized chip architecture was obtained, on which six individual chambers were integrated. At least 1680 microelectrodes were patterned within any one chamber. Alternating current signals have been used to manipulate and align cells, and most cells were aligned as cell-cell twins. High-intensity (~10~3V/cm) electric pulses were used to fuse the aligned cell-cell twins. The fusion efficiency was about 40%, which was much higher than that in traditional chemical method (less than l‰) and electrofusion methods (less than 5%).
机译：通过使用MEMS技术制造了基于微电极阵列的细胞电融合芯片。由于两个反微电极之间的距离很短，因此该芯片上的工作电压仅为传统细胞电融合方法的1 / 100-1 / 20。仿真方法被用来分析芯片上的电场分布并优化微电极的结构。结果表明，微电极的长度和宽度以及两个微电极在水平和垂直方向上的距离都会影响电场的强度和分布。因此，获得了优化的芯片架构，在该架构上集成了六个独立的腔室。在任何一个室内至少有1680个微电极被图案化。交流信号已被用于操纵和对齐细胞，并且大多数细胞被对齐为细胞-细胞双胞胎。高强度（〜10〜3V / cm）的电脉冲被用来融合对准的双胞胎。熔合效率约为40％，远高于传统化学方法（小于l‰）和电熔方法（小于5％）。

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