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首页> 外文期刊>Animal Genetics >Genomic structure, chromosomal localization and expression profile of a porcine long non-coding RNA isolated from long SAGE libraries
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Genomic structure, chromosomal localization and expression profile of a porcine long non-coding RNA isolated from long SAGE libraries

机译：从长SAGE文库中分离的猪长非编码RNA的基因组结构，染色体定位和表达谱

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Long non-coding RNA (long ncRNA) is a novel class of ncRNA that may be involved in critical cellular processes. A considerable number of mammalian long ncRNAs have now been isolated but only a small number of these nucleic acids have been functionally well characterized. In this study, to determine the structure, regulation and function of long ncRNA in pigs, TncRNA was isolated from this mammal and its potential function during pig foetus development was identified. We anticipated that this would provide new insights into functional genomic studies in the pig. Using LongSAGE libraries generated from Chinese indigenous Tongcheng and Landrace pigs at three prenatal stages, a novel porcine long ncRNA was identified, TncRNA, which was found to be differentially expressed during myogenesis. The full-length cDNA for this gene is 3409 bp, and it harbours a typical polyadenylation signal sequence located 18 bp upstream from the 3' poly (A) tail. Genomic sequence analysis showed that pig TncRNA is alternatively spliced and several transcripts were detected. Using the INRA-University of Minnesota porcine radiation hybrid panel, TncRNA was assigned to SSC2 and found to be closely linked to the microsatellite marker SW256. Porcine TncRNA was found to be expressed in all tissues examined but in variable amounts. Comparisons between the expression profiles of TncRNA at different development stages in Tongcheng and Landrace pigs revealed up-regulation of this molecule in prenatal skeletal muscle, and differential expression in 90-day-old foetal skeletal muscle between these two pig breeds. This is the first report to describe a long ncRNA in pig. Moreover, the distinct expression pattern and structure of porcine TncRNA suggest that it performs complex and critical functions during foetal development.
机译：长非编码RNA（长ncRNA）是一类新型的ncRNA，可能参与关键的细胞过程。现在已经分离出许多哺乳动物的长ncRNA，但是只有少数这些核酸在功能上得到了很好的表征。在这项研究中，为了确定猪中长ncRNA的结构，调控和功能，从该哺乳动物中分离了TncRNA，并鉴定了其在猪胎儿发育过程中的潜在功能。我们预计这将为猪的功能基因组研究提供新的见识。利用在三个产前阶段从中国本土的通城猪和长白猪产生的LongSAGE文库，鉴定了一种新型的猪长ncRNA，即TncRNA，发现它在肌发生过程中表达差异。该基因的全长cDNA为3409 bp，它具有一个典型的聚腺苷酸化信号序列，位于3'poly（A）尾部上游18 bp。基因组序列分析表明，猪TncRNA被剪接，并检测到一些转录本。使用明尼苏达州INRA大学的猪辐射混合研究小组，将TncRNA分配给SSC2，并发现其与微卫星标记SW256紧密相连。发现猪TncRNA在所有检查的组织中表达，但数量可变。铜城猪和长白猪在不同发育阶段的TncRNA表达谱之间的比较表明，该分子在产前骨骼肌中表达上调，并且在这两种猪的90天龄胎儿骨骼肌中表达差异。这是描述猪中长ncRNA的第一个报告。此外，猪TncRNA的独特表达模式和结构表明它在胎儿发育过程中执行复杂而关键的功能。

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