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首页> 外文期刊>Immunopharmacology and immunotoxicology >Evaluation of the skin sensitization potential of chemicals in THP-1/keratinocyte co-cultures
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Evaluation of the skin sensitization potential of chemicals in THP-1/keratinocyte co-cultures

机译：THP-1 /角质形成细胞共培养物中化学物质对皮肤的致敏性评估

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Many attempts have been made to develop in vitro sensitization tests that employ dendritic cells (DCs), DC-like cell lines or keratinocytes. The aim of the present investigation was to establish a co-culture of THP-1 cells and keratinocytes for evaluation of skin sensitization potential of chemicals. Co-cultures were constructed by THP-1 cells cultured in lower compartments and keratinocytes cultured in upper compartments of cell culture inserts. After 24h exposure to sensitizers (2, 4-dinitrochlorobenzene, p-phenylenediamine, formaldehyde, nickel sulfate, isoeugenol and eugenol) and non-sensitizers (sodium lauryl sulfate, benzalkonium chloride and lactic acid), the expression of CD86 and CD54 on THP-1 cells were evaluated by flow cytometry, and cell viabilities were determined. The sensitizers induced the augmentation of CD86 and CD54 expression, but the non-sensitizers had no significant effect. Compared with mono-cultures of THP-1 cells, the augmentation of CD86 and CD54 could be detected even at a non-toxic concentration of sensitizers in THP-1 cell/keratinocyte co-cultures. Moreover, isoeugenol was distinguished as a sensitizer in co-cultures, but failed to be identified in mono-cultures. These results revealed that the co-cultures of THP-1 cells and keratinocytes were successfully established and suitable for identifying sensitizers using CD86 and CD54 expression as identification markers.
机译：已经进行了许多尝试来开发利用树突细胞（DC），DC样细胞系或角质形成细胞的体外敏化测试。本研究的目的是建立THP-1细胞和角质形成细胞的共培养物，以评估化学物质对皮肤的敏感性。通过在细胞培养插入物的下部隔室中培养的THP-1细胞和在细胞培养插入物的上部隔室中培养的角质形成细胞来构建共培养物。暴露于敏化剂（2、4-二硝基氯苯，对苯二胺，甲醛，硫酸镍，异丁香酚和丁子香酚）和非敏化剂（十二烷基硫酸钠，苯扎氯铵和乳酸）24小时后，THP-上CD86和CD54的表达通过流式细胞术评估1个细胞，并测定细胞活力。敏化剂诱导CD86和CD54表达的增加，但非敏化剂没有明显的作用。与THP-1细胞的单培养相比，即使在THP-1细胞/角质形成细胞共培养物中使用无毒浓度的敏化剂，也可以检测到CD86和CD54的增加。此外，异丁香酚在共培养物中被认为是敏化剂，但在单培养物中未能被鉴定。这些结果表明，成功建立了THP-1细胞和角质形成细胞的共培养物，并适于使用CD86和CD54表达作为鉴定标记物来鉴定敏化剂。

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