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首页> 外文期刊>Indian journal of clinical biochemistry: IJCB >Synthesis of Gold Nanoparticles Coated with Aspartic Acid and Their Conjugation with FVIII Protein and FVIII Antibody
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Synthesis of Gold Nanoparticles Coated with Aspartic Acid and Their Conjugation with FVIII Protein and FVIII Antibody

机译：天冬氨酸包覆的金纳米颗粒的合成及其与FVIII蛋白和FVIII抗体的缀合

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摘要

Carboxylate-modified gold nanoparticles (GNPs) were synthesized in a simple one-step process based on the reduction of tetrachloroauric acid by aspartic acid in water. GNPs were identified by UV-Vis spectros-copy, dynamic light scattering (DLS) and transmission electron microscopy. Conjugation of protein molecules with functionalized nanoparticles was performed through electrostatic interaction. The GNP-protein conjugates were characterized by gel electrophoresis. The interaction between functionalized GNPs and protein molecules lead to conformational transition of protein structure after conjugation of protein with GNPs. This process was investigated by fluorescence spectroscopy and circular dichroism spectroscopy.
机译：羧酸修饰的金纳米颗粒（GNP）是基于水中的天冬氨酸将四氯金酸还原的简单一步法合成的。通过紫外线-可见光谱，动态光散射（DLS）和透射电子显微镜鉴定了GNP。蛋白分子与功能化的纳米颗粒的缀合通过静电相互作用进行。通过凝胶电泳对GNP-蛋白缀合物进行表征。蛋白质与GNP结合后，功能化GNP与蛋白质分子之间的相互作用导致蛋白质结构的构象转变。通过荧光光谱和圆二色性光谱研究了该过程。

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