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首页> 外文期刊>Indian Journal of Agricultural Biochemistry >Detection and Analysis of a Possible DNA Polymerase A, Gene in Zea mays L.
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Detection and Analysis of a Possible DNA Polymerase A, Gene in Zea mays L.

机译：玉米中可能的DNA聚合酶A，基因的检测和分析

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摘要

X family DNA polymerases are single polypeptide enzymes essential for short patch DNA synthesis and repair. DNA polymerase X is the only member of the family reported in plants. It is involved in base excision repair and translesion DNA synthesis. Objective of this study was to search for and analysis of DNA polymerase X (pol X) in Zea mays. The protein sequence of DNA polymerase X from Japonica rice (Oryza sativa) was used for BLAST algorithm with maize reference protein sequences to find out orthologous protein. It indicated presence of one uncharacterised protein of maize (LOC100383279) which was found to have high homology with rice pol X. Conserved domain search showed presence of BRCT (Breast cancer susceptibility protein A-1 C-terminal domain), dRP (5'-deoxyribosephosphate) lyase domain in addition to polymerase domain in the uncharacterised enzyme of maize. Homology of DNA sequence between rice and maize DNA polymerase was found in Southern blot. Variable expression of the protein was detected during seed development by Western blot. Result suggested presence of 61 kDa DNA polymerase X protein in maize plant.
机译：X家族DNA聚合酶是短片段DNA合成和修复必不可少的单一多肽酶。 DNA聚合酶X是植物中报告的唯一家族成员。它参与碱基切除修复和病灶DNA合成。这项研究的目的是寻找和分析玉米中的DNA聚合酶X（pol X）。将粳稻（Oryza sativa）的DNA聚合酶X的蛋白质序列与玉米参考蛋白质序列一起用于BLAST算法，以找出直系同源蛋白质。这表明存在一种未鉴定的玉米蛋白（LOC100383279），该蛋白与水稻pol X具有高度同源性。保守结构域搜索显示存在BRCT（乳腺癌敏感性蛋白A-1 C末端结构域），dRP（5'-除玉米未鉴定的酶中的聚合酶结构域外，还包含脱氧核糖磷酸）裂解酶结构域。在Southern印迹中发现水稻和玉米DNA聚合酶之间的DNA序列同源性。通过蛋白质印迹在种子发育过程中检测到蛋白质的可变表达。结果表明玉米植株中存在61 kDa DNA聚合酶X蛋白。

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