...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>In Vitro Cellular and Development Biology. Plant: Journal of the Tissue Culture Association >Efficient plant regeneration via somatic embryogenesis and organogenesis from the explants of Catharanthus roseus
【24h】

Efficient plant regeneration via somatic embryogenesis and organogenesis from the explants of Catharanthus roseus

机译：长春花外植体通过体细胞胚发生和器官发生有效地进行植物再生

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

High-frequency plant regeneration of C. roseus cv. ‘little bright eye’ via somatic embryogenesis and organogenesis from five out of six explants was standardized. Two factors were found to be important for regeneration: (1) the type of explants, and (2) the combination and concentrations of plant growth regulators. The highest regeneration percentage through somatic embryogenesis was obtained from mature zygotic embryo in MS medium supplemented with 7.5 μM of thidiazuron (TDZ). The mature embryo also regenerated efficiently via organogenesis in MS medium supplemented with either 2.5 μM TDZ or 5.3 μM α-naphthalene acetic acid (NAA) and 2.2 μM 6-benzylaminopurine (BA). Hypocotyl and cotyledon did not induce somatic embryogenesis and organogenesis in TDZ-containing medium but gave a maximum percentage of shoots in MS medium supplemented with 5.3 μM NAA and 2.2 μM BA. Stem nodes and meristem tips showed better regeneration via organogenesis in the medium supplemented with NAA and BA and in lower concentrations of TDZ.
机译：C.roseus cv的高频植物再生。标准化了来自六分之三的外植体中通过体细胞胚发生和器官发生产生的“小眼睛”。发现有两个因素对再生很重要：（1）外植体的类型，（2）植物生长调节剂的组合和浓度。通过体细胞胚发生的最高再生百分比是从补充有7.5μM噻唑隆（TDZ）的MS培养基中的成熟合子胚获得的。成熟的胚胎还可以通过器官形成在补充有2.5μMTDZ或5.3μMα-萘乙酸（NAA）和2.2μM6-苄基氨基嘌呤（BA）的MS培养基中有效地再生。下胚轴和子叶在含TDZ的培养基中不诱导体细胞胚发生和器官发生，但在补充了5.3μMNAA和2.2μMBA的MS培养基中芽的百分率最高。茎节和分生组织尖端在补充NAA和BA的培养基中以及在较低的TDZ浓度下，通过器官发生表现出更好的再生能力。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号