...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>In Vitro Cellular and Development Biology. Plant: Journal of the Tissue Culture Association >Efficient propagation and rooting of three citrus rootstocks using different plant growth regulators
【24h】

Efficient propagation and rooting of three citrus rootstocks using different plant growth regulators

机译：使用不同的植物生长调节剂对三种柑橘砧木进行有效繁殖和生根

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The influence of various basal medium and plant growth regulators on the efficient micropropagation of nodal explants from mature trees of alemow, sour orange, and ‘Cleopatra’ mandarin citrus rootstocks was studied. All three citrus rootstock shoot cultures showed a preference for high-salt media, like Murashige and Skoog or Driver and Kuniyuki Walnut medium. Several combinations of N 6-benzyladenine (BA) and adenine (AD), kinetin (KIN) or gibberellic acid (GA) were tested to optimize the shoot proliferation phase. BA/GA combinations improved the proliferation of all the rootstocks studied, especially alemow. The addition of BA and AD to the culture medium improved shoot proliferation in sour orange and ‘Cleopatra’ mandarin in the same way as BA and GA. The addition of different combinations of BA/KIN did not result in further improvement of any of the studied variables. The transfer of in vitro shoots to rooting media, containing different concentrations of indolebutyric acid (IBA) and indoleacetic acid (IAA), resulted in regeneration of complete plantlets. Alemow and ‘Cleopatra’ mandarin shoots rooted well using these plant growth regulators; however, all combinations of IBA and IAA tested resulted in very low rooting percentages in sour orange. To improve rooting in sour orange and ‘Cleopatra’ mandarin, different combinations of naphthaleneacetic acid (NAA) and IBA were tested. All NAA/IBA combinations produced higher rooting percentages than did the IBA/IAA combinations, and in sour orange nearly 100 % of explants developed roots. An efficient and simple protocol for the micropropagation of three citrus rootstocks, alemow, ‘Cleopatra’ mandarin, and sour orange, by culturing nodes from mature plants, has been established.
机译：研究了各种基础培养基和植物生长调节剂对来自成熟的淡水榄，酸橙和“埃及艳后”柑桔柑橘砧木的节外植体有效微繁的影响。三种柑橘类砧木芽培养物均显示出对高盐培养基的偏爱，例如Murashige和Skoog或Driver和Kuniyuki Walnut培养基。测试了N 6-苄基腺嘌呤（BA）和腺嘌呤（AD），激动素（KIN）或赤霉素（GA）的几种组合，以优化芽的增殖期。 BA / GA组合可改善所研究的所有砧木的繁殖，尤其是淡紫色。向培养基中添加BA和AD的方式与BA和GA相同，可改善酸橙和“埃及艳后”普通话中的枝条增殖。 BA / KIN的不同组合的添加未导致任何研究变量的进一步改善。体外芽转移到含有不同浓度的吲哚丁酸（IBA）和吲哚乙酸（IAA）的生根培养基中，导致完整植株再生。使用这些植物生长调节剂，Alemow和“ Cleopatra”普通话枝条扎根良好;但是，测试的IBA和IAA的所有组合导致酸橙中生根的百分比非常低。为了改善酸橙和“埃及艳后”橘子的生根，对萘乙酸（NAA）和IBA的不同组合进行了测试。所有NAA / IBA组合产生的生根百分比均高于IBA / IAA组合，并且在酸橙中，近100％的外植体形成了根。通过培养成熟植物的结节，已经建立了一种有效且简单的方案，可对三种柑橘类砧木，lem菜，“埃及艳后”普通话和酸橙进行微繁殖。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号