...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>In Vitro Cellular and Development Biology. Plant: Journal of the Tissue Culture Association >Factors affecting micropropagation and acclimatization of an elite clone of Eucalyptus tereticornis Sm.
【24h】

Factors affecting micropropagation and acclimatization of an elite clone of Eucalyptus tereticornis Sm.

机译：影响细桉桉Sm克隆无性繁殖和适应的因素。

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Several factors influencing micropropagation of a selected elite clone of Eucalyptus tereticornis Sm. were investigated. Amongst different cytokinins tested, 6-benzyleadenine proved to be the most effective cytokinin for shoot multiplication and elongation. The initial size of the shoot clump (inoculum) also influenced shoot multiplication and elongation. The number of shoots proliferated per culture vessel were significantly higher (342 shoots per culture vessel) when larger shoot clumps (15–20 shoots) were inoculated, compared to smaller shoot clumps (4–5 shoots), which resulted in a reduced shoot proliferation rates (245 shoots per culture vessel). However, the number of elongated shoots (65 per culture vessel) and shoot length (5.23 cm) were higher in cultures which were inoculated with smaller shoot clumps in comparison to those cultures which were inoculated with larger shoot clumps (54 shoots per culture vessel with shoot length of 4.17 cm). The maximum number of rooted shoots (80.7 %) was obtained on one fourth-strength MS medium supplemented with 5.0 μM indolebutyric acid. The number of shoots proliferated, elongated, rooting frequency, and subsequent survival of plants after acclimatization were higher in cultures incubated under photosynthetically active radiation (PAR) compared to those incubated under cool fluorescent lights (CFL). Osmotic potential of the sap and chlorophyll content of cultures incubated under PAR were also higher than those incubated under CFL. Following transfer of plants to soil, inoculation with a suspension of Bacillus subtilis (plant growth-promoting bacterium) increased the survival rate of plants by 10 %, yielding successful transfer of 84 % of plants. Random amplified polymorphic DNA and inter simple sequence repeat analyses indicated a high level of clonal uniformity amongst regenerated plants and also with that of the mother plant.
机译：几种影响特选桉树细小克隆无性繁殖的因素。被调查了。在测试的不同细胞分裂素中，6-苄基腺嘌呤被证明是最有效的细胞分裂素，用于芽繁殖和伸长。芽丛的初始大小（接种物）也影响芽的繁殖和伸长。接种较大的丛枝（15–20个芽）时，与较小的丛枝（4–5个芽）相比，每个培养皿中繁殖的芽数显着更高（每个培养皿中为342个芽），导致芽增殖减少率（每个培养皿245个芽）。但是，与接种较大芽苗的培养物相比，接种较大芽苗的培养物中的细长芽数（每个培养皿65个）和芽长（5.23 cm）要高（与接种较大芽苗的培养物相比，每个培养皿54个芽）。芽长4.17厘米）。在一种补充有5.0μM吲哚丁酸的四强度MS培养基上获得了最大的生根芽数（80.7％）。在光合作用活性辐射（PAR）下培养的培养物中，与在冷荧光灯（CFL）下培养的培养相比，适应后植物的增殖，伸长，生根频率和随后的存活枝数更高。在PAR下培养的培养液的汁液渗透势和叶绿素含量也高于在CFL下培养的渗透液。将植物转移到土壤后，接种枯草芽孢杆菌（促进植物生长的细菌）悬浮液可使植物存活率提高10％，成功转移84％的植物。随机扩增的多态性DNA和简单的序列重复分析表明，再生植物之间以及与母本植物之间的克隆均一性很高。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号