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首页> 外文期刊>In Vitro Cellular and Development Biology. Plant: Journal of the Tissue Culture Association >Efficient plant regeneration via somatic embryogenesis in bulbing fennel using immature flower explants
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Efficient plant regeneration via somatic embryogenesis in bulbing fennel using immature flower explants

机译：使用未成熟的花外植体通过鳞茎茴香的体细胞胚发生有效的植物再生。

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摘要

In order to optimize plant regeneration techniques for bulbing fennel [Foeniculum vulgare Mill. subsp. vulgare var. azoricum (Mill.) Thell] via somatic embryogenesis, three different media were tested on four Sicilian fennel ecotypes derived from seed collections. Plant regeneration was obtained through the use of immature inflorescences as explants. Through comparison of different auxins, naphthoxyacetic acid resulted in high-level induction of embryogenic callus at the lowest concentration (4 μM), in combination with 6-benzylaminopurine or thidiazuron. After germination of somatic embryos, regenerated plantlets were recovered on medium containing half-strength Murashige and Skoog salts and vitamins in the absence of plant growth regulators. Regenerated plants were successfully acclimatized (70 %) in a growth chamber prior to transplantation into an open field. The plants appeared phenotypically normal, and both genetic stability and uniformity were confirmed by random amplified polymorphism DNA analysis. The results represent a significant advance on previous reports, due to the high embryogenic ability of immature inflorescence from different ecotypes and the high percentage of regenerated plantlets acclimatized in ex vitro conditions.
机译：为了优化用于鳞茎茴香的植物再生技术[Foeniculum vulgare Mill。亚种俗变通过体细胞胚发生，对三种不同的培养基对源自种子集合的四种西西里茴香生态型进行了测试。通过使用未成熟的花序作为外植体获得植物再生。通过比较不同的植物生长素，萘氧基乙酸与6-苄基氨基嘌呤或噻二唑联合以最低浓度（4μM）高水平诱导胚胎发生的愈伤组织。体细胞胚萌发后，在没有植物生长调节剂的情况下，在含有半强度Murashige和Skoog盐和维生素的培养基上回收再生的幼苗。在移植到开放田地之前，再生植物已​​在生长室中成功地适应了环境（70％）。这些植物在表型上看起来是正常的，并且通过随机扩增的多态性DNA分析证实了遗传稳定性和均匀性。由于来自不同生态类型的未成熟花序的高胚发生能力以及离体条件下适应的再生小植株的百分比很高，因此该结果代表了先前报道的重大进展。

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