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首页> 外文期刊>In Vitro Cellular and Development Biology. Plant: Journal of the Tissue Culture Association >Soluble carbohydrate content of soybean (Glycine max (L.) Merr.) somatic and zygotic embryos during development.
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Soluble carbohydrate content of soybean (Glycine max (L.) Merr.) somatic and zygotic embryos during development.

机译：发育期间大豆（Glycine max（L.）Merr。）体细胞和合子胚的可溶性碳水化合物含量。

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摘要

During development, zygotic embryos of soyabeans cv. Jack accumulated trace amounts of fructose, galactose and galactinol, whereas somatic embryos contained only trace amounts of galactose. Somatic embryos accumulated much higher glucose levels thanzygotic embryos. Both somatic and zygotic embryos contained low levels of sucrose, myoinositol and pinitol. Raffinose and stachyose accumulated in the late developmental stages of zygotic embryos, but only stachyose accumulated in the late-stage somaticembryos. Zygotic embryos contained low total lipid levels up to 50 days after flowering (DAF) and then the levels increased to 16% by 55 DAF and 21% at 65 DAF. Somatic embryos had low levels of total lipids throughout development with a maximum of only4.7%. Zygotic embryos contained about 40% protein throughout development, while the protein concentration of somatic embryos decreased from 44 to 25% as maturation approached. These studies demonstrate that the composition of Jack zygotic embryos is similar to that described for other cultivars during development while the somatic embryo composition and size is significantly different. It is suggested that the low somatic embryo germination rate often noted might be due to the abnormal development as shown by a composition different from that of mature zygotic embryos. The low concentration of the raffinose series sugars might be important factors.
机译：在发育过程中，大豆的合子胚简历。杰克积聚了痕量的果糖，半乳糖和半乳糖醇，而体细胞胚仅含有痕量的半乳糖。体细胞胚比合子胚积累了更高的葡萄糖水平。体细胞和合子胚均含有低水平的蔗糖，肌醇和松醇。棉子糖和水苏糖在合子胚的发育后期积累，但仅水苏糖在体细胞胚的后期积累。合子胚在开花后长达50天（DAF）时总脂质水平较低，然后在55 DAF时升高至16％，在65 DAF时升高至21％。在整个发育过程中，体细胞胚的总脂质水平较低，最高仅为4.7％。合子胚在整个发育过程中均包含约40％的蛋白质，而随着成熟的临近，体细胞胚的蛋白质浓度从44％降至25％。这些研究表明，杰克合子胚的组成与发育过程中其他品种的描述相似，而体细胞胚的组成和大小却存在显着差异。有人指出，经常注意到的体细胞胚发芽率低可能是由于发育异常所致，其组成不同于成熟的合子胚。棉子糖系列糖的低浓度可能是重要的因素。

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