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首页> 外文期刊>Indian Journal of Fisheries >Biochemical composition of the ovary and partial characterisation of yolk protein vitellin in Metapenaeus monoceros (Fabricius, 1798)
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Biochemical composition of the ovary and partial characterisation of yolk protein vitellin in Metapenaeus monoceros (Fabricius, 1798)

机译：Metapenaeus monoceros卵巢的生化组成和卵黄蛋白卵黄蛋白的部分表征（Fabricius，1798年）

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摘要

Yolk protein vitellin in 'speckled shrimp' Metapenaeus monoceros was partially characterised employing polyacrylamide gel electrophoresis (PAGE). There was an increase in the level of expression of vitellin in the ovary, with the advancement of maturation. The vitellin fraction was not expressed in immature and spent ovaries. Molecular weight of vitellin was 326 kDa. Confirmation of the vitellin band by selective staining showed that the yolk protein was a lipo-glyco-carotenoprotein possessing a calcium moiety. A protein fraction with molecular weight 270 kDa, which was a lipoprotein, was expressed in ovaries of all maturity stages as well as in testis. The major biochemical constituents of mature yolk of M. monoceros on dry weight basis were protein (59.36+-1.18%), lipid (31.23+-2.98%) and carbohydrates (2.97+-0.81%). Carotenoids accounted for 0.70+-0.81% wet weight of yolk.
机译：利用聚丙烯酰胺凝胶电泳（PAGE）部分表征了“斑点虾” Metapenaeus monoceros中的卵黄蛋白卵黄蛋白。随着成熟的发展，卵巢中卵黄蛋白的表达水平增加。卵黄蛋白部分未在未成熟和用过的卵巢中表达。卵黄蛋白的分子量为326 kDa。通过选择性染色对卵黄蛋白带的确认表明卵黄蛋白是具有钙部分的脂-糖-胡萝卜素蛋白。在所有成熟阶段的卵巢以及睾丸中都表达了分子量为270 kDa的蛋白质级分，它是一种脂蛋白。以干重为基础，单核甲烷八叠球成熟蛋黄的主要生化成分是蛋白质（59.36±1.18％），脂质（31.23±2.98％）和碳水化合物（2.97±0.81％）。类胡萝卜素占蛋黄湿重的0.70±0.81％。

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