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首页> 外文期刊>Indian Journal of Fisheries >Isolation and characterisation of extreme halophiles Halomonas aquamarina and Halomonas marina from trigger fish, Abalistes stellaris (Bloch & Schneider, 1801)
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Isolation and characterisation of extreme halophiles Halomonas aquamarina and Halomonas marina from trigger fish, Abalistes stellaris (Bloch & Schneider, 1801)

机译:从触发鱼Abalistes stellaris的极端嗜盐菌嗜盐嗜盐菌和嗜盐梭菌的分离和鉴定(Bloch&Schneider,1801)

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摘要

Screening of bacteria from the skin, gills and gut of the marine triggerfish, Abalistes stellaris (Bloch & Schneider, 1801), collected from Vizhinjam, Kerala, India, led to the isolation of 8 halophilic bacterial strains. The isolates were able to grow optimally in culture media with 5-15% salt content. Of these, 3 extremely halophilic bacterial isolates that grew in 20-25% of salt were selected for genotypic characterisation. Bacterial strain, IJ1, isolated from skin, and strains, IJ5 and IJ6, isolated from gut of A. stellaris grew optimally at pH 7.0 and 5-15% NaCl at 35 °C. The cells were Gram negative short rods. According to the phenotypic characteristics and comparative partial 16SrRNA sequence analysis, the strain IJ1 was identified as Halomonas marina strain DSM 4741 (GenBank Accession Number: KC599209) and strains IJ5 and IJ6 as Halomonas aquamarina (GenBank Accession Numbers: KC620376 and KC599210 respectively). H. aquamarina has been reported for the first time from any environment in India and both the strains have been deposited in the microbial repository of National Bureau of Agriculturally Important Microorganisms (NBAIM), Mau, India.
机译:从印度喀拉拉邦Vizhinjam收集的海洋触发鱼Abalistes stellaris(Bloch&Schneider,1801)的皮肤,腮和肠中筛选细菌,从而分离出8种嗜盐细菌菌株。分离物能够在含盐量为5-15%的培养基中最佳生长。其中,选择了生长在20%至25%的盐中的3种极端嗜盐细菌分离株进行基因型鉴定。从皮肤分离的细菌菌株IJ1和从恒星肠菌的肠道分离的IJ5和IJ6菌株在35°C的pH 7.0和5-15%NaCl下生长最佳。细胞是革兰氏阴性短杆。根据表型特征和比较的16SrRNA部分序列分析,菌株IJ1被鉴定为滨海假单胞菌菌株DSM 4741(GenBank登录号:KC599209),菌株IJ5和IJ6被鉴定为海藻单胞菌(GenBank登录号:KC620376和KC599210)。在印度的任何环境中都首次报道了海藻嗜血杆菌,并且两种菌株都已被存放在印度茂的国家农业重要微生物局(NBAIM)的微生物库中。

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