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首页> 外文期刊>Indian Journal of Small Ruminants >A rapid method for estimating total protein in goat milk by Amido black 10B dye.
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A rapid method for estimating total protein in goat milk by Amido black 10B dye.

机译：一种氨基10B染料快速估算山羊奶中总蛋白的方法。

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摘要

The basic mechanism behind the dye binding method is that insoluble precipitate forms rapidly as acid dye is combined with proteins in an acidified buffer solution. Amido Black 10B is disulfonic acid commonly used in milk protein estimation. A standard curve was plotted between total protein percentage (Kjeldahl) and the difference between the optical densities of the blank and the sample and protein content by dye binding method was estimated by the standard curve. The protein percentage determined by the Kjeldahl and dye-binding methods showed significant (P 0.01) correlation coefficient of 0.989. The difference between the two methods of protein estimation was not significant and ranged from - 0.35% to +0.33% protein, revealing that Amido Black 10B dye method of determining protein in milk is as accurate as the Kjeldahl procedure. So Amido Black 10B dye binding method is reliable and convenient for routine analysis of goat milk protein.
机译：染料结合方法的基本原理是，当酸性染料与蛋白质在酸化的缓冲溶液中结合时，不溶的沉淀物会迅速形成。酰胺黑10B是通常用于乳蛋白评估的二磺酸。在总蛋白百分率（凯氏定氮）之间绘制标准曲线，并通过标准曲线估计空白和样品的光密度与通过染料结合法的蛋白含量之间的差异。通过凯氏定氮法和染料结合法测定的蛋白质百分比显示出显着的（P 0.01）相关系数0.989。两种蛋白质估算方法之间的差异不显着，范围在-0.35％至+ 0.33％蛋白质之间，这表明测定牛奶中蛋白质的Amido Black 10B染料方法与凯氏定氮法一样准确。因此Amido Black 10B染料结合方法可靠，方便，可用于山羊奶蛋白的常规分析。

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