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首页> 外文期刊>Indian Journal of Small Ruminants >GENETIC POLYMORPHISM OF GROWTH HORMONE GENE IN NATIVE SHEEPBREEDS OF INDIA
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GENETIC POLYMORPHISM OF GROWTH HORMONE GENE IN NATIVE SHEEPBREEDS OF INDIA

机译：印度本地种生长激素基因的遗传多态性

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摘要

Genetic polymorphism of growth hormone gene at locus A1575G and A781G was studied for 9 sheep breeds of different agro-ecological regions of India. A total of 324 random blood samples of Indian sheep breeds were analyzed by PCR- RFLP (Polymerase chainreaction- Restriction fragment length polymorphism) technique. Restriction digestion analysis atA781 G locus revealed two allelic variants (A= 0.6016 and B= 0.3983) and two genotypes (AA= 0.2032, AB= 0.7968). At A1575G locus, all individuals were homozygous (CC) in all 9 breeds. The allelic frequency differences for both alleles across the Indian breeds, irrespective of their geographic distribution, colour pattern and utility traits, were statistically non-significant. The average heterozygosity was 0.4712. These findings reveal that substantial level of genetic variation exists at A781G locus and adds substance to search for QTL (quantitative trait locus) for growth trait in Indian sheep breeds.
机译：研究了印度不同农业生态区的9个绵羊品种的A1575G和A781G位点生长激素基因的遗传多态性。通过PCR-RFLP（聚合酶链反应-限制性片段长度多态性）技术分析了324个印度绵羊品种的随机血液样本。在A781 G位点的限制性酶切分析显示了两个等位基因变体（A = 0.6016和B = 0.3983）和两个基因型（AA = 0.2032，AB = 0.7968）。在A1575G基因座上，所有9个品种的所有个体均为纯合子（CC）。整个印度品种中两个等位基因的等位基因频率差异，无论其地理分布，颜色模式和实用性状如何，在统计上均无统计学意义。平均杂合度为0.4712。这些发现表明，A781G基因座存在大量遗传变异，并为寻找印度绵羊品种生长性状的QTL（定量性状基因座）增加了物质。

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