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首页> 外文期刊>Indian Journal of Small Ruminants >Incidence of Mycobacterium avium subspecies Paratuberculosis in Patanwadi sheep.
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Incidence of Mycobacterium avium subspecies Paratuberculosis in Patanwadi sheep.

机译：Patanwadi羊中鸟分枝杆菌亚种副结核病的发病率。

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摘要

Incidence of Mycobacterium avium subspecies paratuberculosis (MAP) infection was estimated in Patanwadi sheep at Livestock Research Station, Sardarkrushinagar (Gujarat), using multiple tests. More than 50.0% of Patanwadi sheep had clinical signs (reduced appetite, diarrhoea, weight loss, emaciation, rough wool coat and leathery skin) of paratuberculosis. Of 450 sheep, 133 were screened to estimate bio-load of MAP, wherein 50.3% were positive by indigenous ELISA. Faeces of 50 sheep positive in i-ELISA were screened by microscopy and 13 (26.0%) were positive for acid fast bacilli (AFB) indistinguishable from MAP. Of 13 AFB positive faecal samples in direct microscopy, 3 (23.0%) were also positive on IS900 faecal PCR. Bio-load of MAP (bactremia) was estimated in 50 i-ELISA positive sheep by IS900 blood PCR and 8 (16.0%) were found positive. This is the first study on incidence of MAP in Patanwadi sheep.
机译：使用多项测试，在萨尔达库鲁奇纳加尔（古吉拉特邦）家畜研究站的帕坦瓦迪羊中估计了禽分枝杆菌副结核副杆菌（MAP）感染的发生率。超过50.0％的Patanwadi绵羊患有副结核病的临床体征（食欲降低，腹泻，体重减轻，消瘦，羊毛大衣和皮革质皮肤）。在450只绵羊中，筛选了133只绵羊以估计MAP的生物负荷，其中50.3％通过本地ELISA呈阳性。通过显微镜筛选了50只在i-ELISA中呈阳性的绵羊的粪便，其中13例（26.0％）的耐酸杆菌（AFB）与MAP不能区分。在直接显微镜下的13份AFB阳性粪便样本中，有3份（23.0％）在IS900粪便PCR上也呈阳性。通过IS900血液PCR评估了50只i-ELISA阳性绵羊中MAP（细菌血症）的生物负荷，并且发现8只（16.0％）为阳性。这是帕坦瓦迪绵羊中MAP发病率的第一项研究。

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