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首页> 外文期刊>Brain research >Involvement of phosphatidylinositol 3-kinase and insulin-like growth factor-I in YXLST-mediated neuroprotection.
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Involvement of phosphatidylinositol 3-kinase and insulin-like growth factor-I in YXLST-mediated neuroprotection.

机译：磷脂酰肌醇3-激酶和胰岛素样生长因子-I参与YXLST介导的神经保护作用。

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摘要

In the present study, we examine the neuroprotective role of the external Qi of YXLST in cultured retinal neurons. Primary retinal neuronal cultures were grown from retinas of 0-2-day-old Sprague-Dawley rats. Cultures were treated directly with external Qi of YXLST 30 min prior to H(2)O(2) exposure in most experiments. Cell viability was measured by 3,(4,5-dimethylthiazol-2-yl)2,5-diphenyl-tetrazolium bromide (MTT) assay. Apoptotic cell death was evaluated by the TdT-mediated digoxigenin-dUTP nick-end labeling TUNEL assay, and by DNA laddering analysis. Northern blot analysis was performed to examine the level of insulin-like growth factor-I (IGF-I) gene expression. Phosphatidylinositol 3-kinase (PI3K) assay was performed to study the PI3K activity. The results showed that treatment of external Qi of YXLST significantly attenuated neuronal death that was induced by 24-h exposure to hydrogen peroxide, and greatly inhibited hydrogen peroxide-induced apoptosis. External Qi of YXLST also upregulated IGF-I gene expression and increased PI3K activity. These observations indicate that external Qi-mediated IGF-I expression and PI3K signaling could be one of the mechanisms in neuroprotection by YXLST.
机译：在本研究中，我们检查了在培养的视网膜神经元中YXLST的外部气管的神经保护作用。从0-2天大的Sprague-Dawley大鼠的视网膜生长主要的视网膜神经元培养物。在大多数实验中，在H（2）O（2）暴露之前，用YXLST的外部Qi直接处理培养物30分钟。通过3，（4,5-二甲基噻唑-2-基）2,5-二苯基溴化四唑鎓（MTT）测定法测量细胞活力。通过TdT介导的地高辛配基-dUTP缺口末端标记TUNEL测定和DNA梯形分析评估凋亡细胞的死亡。进行Northern印迹分析以检查胰岛素样生长因子-1（IGF-1）基因表达的水平。进行磷脂酰肌醇3-激酶（PI3K）测定以研究PI3K活性。结果表明，YXLST的外部气虚治疗显着减轻了过氧化氢暴露24 h诱导的神经元死亡，并大大抑制了过氧化氢诱导的细胞凋亡。 YXLST的外部Qi也上调了IGF-I基因表达并增加了PI3K活性。这些观察表明，外部Qi介导的IGF-1表达和PI3K信号传导可能是YXLST对神经保护的机制之一。

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