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首页> 外文期刊>Brain research >Involvement of 5-HT(1B) receptors within the ventral tegmental area in regulation of mesolimbic dopaminergic neuronal activity via GABA mechanisms: a study with dual-probe microdialysis.
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Involvement of 5-HT(1B) receptors within the ventral tegmental area in regulation of mesolimbic dopaminergic neuronal activity via GABA mechanisms: a study with dual-probe microdialysis.

机译：通过GABA机制参与腹侧被盖区5-HT（1B）受体参与中脑边缘多巴胺能神经元活性的调节：一项双探针微透析研究。

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This study was designed to assess the involvement of 5-HT(1B) receptors within the ventral tegmental area (VTA) in the regulation of mesolimbic dopaminergic transmission. Dual-probe microdialysis was performed in freely moving adult Sprague-Dawley rats with one probe within the VTA and the other within the ipsilateral nucleus accumbens (NACC). Drugs were administered into the VTA via retrograde dialysis. Dialysates from both the VTA and the NAC were collected for determination of dopamine (DA) and gamma-aminobutyric acid (GABA) by high-performance liquid chromatography with electrochemical detection. Intra-tegmental infusion of CP 93129 (20, 40, and 80 microM), a 5-HT(1B) receptor agonist, increased extracellular DA concentrations in a concentration-dependent manner not only in the NACC but also in the VTA, indicating increased mesolimbic DA neuron activity. Administration of CP 93129 at 80 microM into the VTA also significantly decreased extracellular GABA concentrations in this region. Co-infusion ofthe 5-HT(1B) receptor antagonist SB 216641 (10 microM), but not the 5-HT(1A) receptor antagonist WAY 100635 (10 microM) or the 5-HT(1D/1A) receptor antagonist BRL 15572 (10 microM), antagonized not only the effects of intra-tegmental CP 93129 (80 microM) on VTA DA and NAC DA but also on VTA GABA. The results suggest that activation of VTA 5-HT(1B) receptors increases mesolimbic DA neuron activities. The increased DA neuron activity may be associated, at least in part, with the 5-HT(1B) receptor-mediated inhibition of VTA GABA release.
机译：这项研究旨在评估5-HT（1B）受体在腹侧被盖区（VTA）参与中脑边缘多巴胺能传递的调节。在自由移动的成年Sprague-Dawley大鼠中进行双探针微透析，其中一个探针位于VTA内，另一个位于同侧伏隔核（NACC）内。药物通过逆行透析进入VTA。收集了来自VTA和NAC的透析液，用于​​通过电化学检测的高效液相色谱法测定多巴胺（DA）和γ-氨基丁酸（GABA）。鞘内输注5-HT（1B）受体激动剂CP 93129（20、40和80 microM）不仅在NACC中而且在VTA中以浓度依赖的方式增加了细胞外DA的浓度，表明增加了中脑边缘DA神经元活性。在VTA中以80 microM的剂量施用CP 93129也显着降低了该区域的细胞外GABA浓度。 5-HT（1B）受体拮抗剂SB 216641（10 microM），5-HT（1A）受体拮抗剂WAY 100635或5-HT（1D / 1A）受体拮抗剂BRL 15572不共注入（10 microM），不仅拮抗了片段内CP 93129（80 microM）对VTA DA和NAC DA的作用，而且还拮抗了VTA GABA的作用。结果表明，激活VTA 5-HT（1B）受体会增加中脑边缘DA神经元的活动。增加的DA神经元活性可能至少部分与5-HT（1B）受体介导的VTA GABA释放抑制有关。

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