Cloning, sequencing and characterization of chicken growth hormone receptor gene

Kumar G. V. P. P. S. Ravi; Brah G. S.; Chaudhary M. L.; Pawar H. N.; Mathur Pranav

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Cloning, sequencing and characterization of chicken growth hormone receptor gene

机译：鸡生长激素受体基因的克隆，测序与鉴定

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In this study 762 bp amplicon of chicken Growth hormone receptor (GHR) gene has been cloned, sequenced and characterized. No polymorphism was identified at the Alu I, Sau 3A I, Pst I, Nsp I, and Hind III sites. The partial exon-5 sequence showed variability at nucleotide positions 8, 15 and 16 amongst the species studied. On phylogenetic analysis the sequence in this study clustered along with the other avian GHR sequences clearly away from the mammalian cluster. At position 8, transition from 0 to A and at 15, 16 and 23 positions transversions from A to T, G to C and T to G respectively, were identified among the sequences. However, the substitutions identified may hardly affect the structure and function of the GHR protein since all different substitutions resulted in amino acids belonging to similar groups.

机译：在这项研究中，已克隆，测序和鉴定了762 bp的鸡生长激素受体（GHR）基因的扩增子。在Alu I，Sau 3A I，Pst I，Nsp I和Hind III位点未发现多态性。在所研究的物种中，外显子5的部分序列在核苷酸的8、15和16位处表现出变异性。在系统发育分析中，本研究中的序列与其他禽类GHR序列明显聚集，远离哺乳动物群。在序列中，分别确定了从0到A的转变，以及从15到16、23和23的转变，分别从A到T，G到C和T到G。但是，由于所有不同的取代导致氨基酸属于相似的基团，因此鉴定出的取代几乎不会影响GHR蛋白的结构和功能。

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来源
《Indian Journal of Poultry Science》 |2009年第1期|共5页
作者
Kumar G. V. P. P. S. Ravi; Brah G. S.; Chaudhary M. L.; Pawar H. N.; Mathur Pranav;
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正文语种 eng
中图分类家禽;
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Methods and Techniques; Molecular Genetics: Biochemistry and Molecular Biophysics;

机译：方法与技术;分子遗传学：生物化学与分子生物物理学;

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1. Cloning, sequencing and characterization of chicken growth hormone receptor gene [J] . Kumar G. V. P. P. S. Ravi, Brah G. S., Chaudhary M. L., Indian Journal of Poultry Science . 2009,第1期

机译：鸡生长激素受体基因的克隆，测序与鉴定
2. Growth hormone down-regulates growth hormone receptor mRNA in chickens but developmental increases in growth hormone receptor mRNA occur independently of growth hormone action. [J] . Mao JN, Cogburn LA, Burnside J Molecular and Cellular Endocrinology . 1997,第2期

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Cloning, sequencing and characterization of chicken growth hormone receptor gene

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