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首页> 外文期刊>Brain research >Anoxia induces matrix shrinkage accompanied by an increase in light scattering in isolated brain mitochondria.
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Anoxia induces matrix shrinkage accompanied by an increase in light scattering in isolated brain mitochondria.

机译：缺氧诱导基质收缩，伴随着孤立的脑线粒体中光散射的增加。

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摘要

It is important to monitor mitochondrial conditions, and light scattering (LS) measurements have been applied to the detection of morphological changes in mitochondria in vivo. Little is known about the morphological and LS responses of brain mitochondria to oxygen withdrawal, a critical factor in cell death. We have therefore investigated the morphological and LS responses of isolated brain mitochondria to anoxia. Anoxia induced an increase in LS, reflecting mitochondrial matrix shrinkage. This response was reversible, but was reduced by adding digitonin, which disrupted the outer membrane selectively. This suggested that integrity of the outer membrane was necessary for the matrix response. We further examined the effects of Mg2+ and ATP on the responses because both exist in cells and modulate the changes in matrix volume. Although Mg2+ and ATP reduced the rates of increase and decrease in LS, respectively, the magnitudes of the increases in LS caused by anoxia stayed at over 80% of the control level (no Mg2+) in the presence of Mg2+ and ATP. This suggested that the increase in LS occurred in cells containing Mg2+ and ATP during anoxia. In contrast, that caused by inhibitors of the electron transport chain was reduced to below 30% of the control level in the presence of Mg2+. The present in vitro study provides a basis for interpretation of LS signals from mitochondria in brain research during oxygen withdrawal.
机译：监测线粒体状况非常重要，并且光散射（LS）测量已应用于体内线粒体形态变化的检测。关于脑线粒体对氧吸收（细胞死亡的关键因素）的形态和LS反应知之甚少。因此，我们研究了孤立的脑线粒体对缺氧的形态和LS反应。缺氧引起LS的增加，反映了线粒体基质的收缩。该反应是可逆的，但通过添加洋地黄皂苷可降低反应性，这可选择性地破坏外膜。这表明外膜的完整性对于基质反应是必需的。我们进一步检查了Mg2 +和ATP对反应的影响，因为两者都存在于细胞中并调节基质体积的变化。尽管Mg2 +和ATP分别降低了LS的增加和减少的速率，但在存在Mg2 +和ATP的情况下，由缺氧引起的LS的增加幅度保持在对照水平的80％以上（无Mg2 +）。这表明缺氧期间LS的增加发生在含有Mg2 +和ATP的细胞中。相反，在存在Mg2 +的情况下，由电子传输链抑制剂引起的释放降低到控制水平的30％以下。目前的体外研究为在吸氧期间脑研究中解释线粒体的LS信号提供了基础。

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