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首页> 外文期刊>Brain research >Morpho-functional characterization of neuronal cells at different stages of maturation in granule cell layer of adult rat dentate gyrus.
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Morpho-functional characterization of neuronal cells at different stages of maturation in granule cell layer of adult rat dentate gyrus.

机译：成年大鼠齿状回的颗粒细胞层中不同成熟阶段神经元细胞的形态功能表征。

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Neurogenesis occurs throughout adult life in dentate gyrus of mammal hippocampus. Therefore, neurons at different stages of electrophysiological and morphological maturation and showing various, if any, synaptic inputs co-exist in the adult granule cell layer, as occurs during dentate gyrus development. The knowledge of functional properties of new neurons throughout their maturation can contribute to understanding their role in the hippocampal function. In this study electrophysiological and morphological features of granule layer cells, characterized as immature or mature neurons, without and with synaptic input, were comparatively described in adult rats. The patch-clamp technique was used to perform electrophysiological recordings, the occurrence of synaptic input evoked by medial perforant pathway stimulation was investigated and synaptic input was characterized. Cells were then identified and morphologically described via detection of biocytin injected through the patch pipette. The neuronal phenotype of recorded cells was assessed by immunohistochemistry and single-cell RT-PCR. Cells with very low capacitance, high input resistance, depolarized resting membrane potential and without synaptic activity were found exclusively at the border of the GCL facing hilus; this type of cell expressed the class III beta-tubulin neuronal marker (mRNA and protein) and did not express a glial marker. Immature neuronal cells with progressively increasing capacitance, decreasing input resistance and resting membrane potential getting more hyperpolarized showed only depolarizing GABAergic synaptic input at first and then also glutamatergic synaptic input. Finally, cells showing electrophysiological, synaptic, and morphological features of mature granule, expressing the mature neuron marker NeuN, were identified.
机译：在整个成年生活中，神经发生发生在哺乳动物海马的齿状回中。因此，在成年的颗粒细胞层中，如在齿状回发育期间所发生的，处于电生理学和形态学成熟的不同阶段并显示出各种突触输入的神经元（如果有的话）共存。新神经元在整个成熟过程中的功能特性知识可有助于理解其在海马功能中的作用。在这项研究中，成年大鼠比较描述了颗粒层细胞的电生理和形态学特征，其特征为不成熟或成熟的神经元，无突触输入。使用膜片钳技术进行电生理记录，研究由内侧穿孔途径刺激引起的突触输入的发生并表征突触输入。然后通过检测通过贴片移液器注射的生物细胞素来鉴定细胞并进行形态学描述。通过免疫组织化学和单细胞RT-PCR评估记录细胞的神经元表型。仅在GCL面对的边界发现了具有极低电容，高输入电阻，去极化的静息膜电位且没有突触活性的细胞。这种类型的细胞表达III类β-微管蛋白神经元标记（mRNA和蛋白质），但不表达神经胶质标记。具有逐渐增加的电容，降低的输入电阻和静息膜电位变得越来越极化的未成熟神经元细胞首先仅对GABA能突触输入起去极化作用，然后对谷氨酸能突触输入起作用。最后，鉴定出表现出成熟颗粒的电生理，突触和形态特征的细胞，该细胞表达成熟神经元标记物NeuN。

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