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首页> 外文期刊>Brain research >Morphological features and electrophysiological properties of serotonergic and non-serotonergic projection neurons in the dorsal raphe nucleus. An intracellular recording and labeling study in rat brain slices.
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Morphological features and electrophysiological properties of serotonergic and non-serotonergic projection neurons in the dorsal raphe nucleus. An intracellular recording and labeling study in rat brain slices.

机译：背脊核中血清素能和非血清素能投射神经元的形态学特征和电生理特性。大鼠脑切片的细胞内记录和标记研究。

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摘要

The morphology and electrophysiological properties of serotonergic and non-serotonergic projection neurons in the dorsal raphe nucleus (DRN) of the rat were examined in frontal brain slices. Biocytin was injected intracellularly into the intracellularly recorded neurons. Then the morphology of the recorded neurons was observed after histochemical visualization of biocytin. The recorded neurons extending their main axons outside the DRN were considered as projection neurons. Subsequently, serotonergic nature of the neurons was examined by serotonin (5-HT) immunohistochemistry. The general form of the dendritic trees is radiant and poorly branching in both 5-HT- and non-5-HT neurons. However, the dendrites of the 5-HT neurons were spiny, whereas those of the non-5-HT neurons were aspiny. The main axons of both 5-HT- and non-5-HT neurons were observed to send richly branching axon collaterals to the DRN, ventrolateral part of the periaqueductal gray and the midbrain tegmentum. In response to weak, long depolarizing current pulses, the 5-HT neurons displayed a slow and regular firing activity. The non-5-HT neurons fired at higher frequencies even when stronger current was injected. Some other differences in electrophysiological properties were also observed between the 5-HT-immunoreactive spiny projection neurons and the 5-HT-immunonegative aspiny projection neurons.
机译：在额叶脑切片中检查了大鼠背沟核（DRN）中的血清素能和非血清素能投射神经元的形态和电生理特性。生物素被细胞内注射到细胞内记录的神经元中。然后在组织化学可视化生物胞素后观察记录的神经元的形态。记录的神经元将其主要轴突延伸到DRN之外被视为投射神经元。随后，通过5-羟色胺（5-HT）免疫组织化学检查了神经元的血清素能性质。树突树的一般形式是辐射状的，在5-HT和非5-HT神经元中分支较弱。然而，5-HT神经元的树突是多刺的，而非5-HT神经元的树突是多刺的。观察到5-HT和非5-HT神经元的主要轴突向DRN，导水管周围灰质的腹侧部分和中脑被盖骨发送丰富的分支轴突侧支。响应弱，长的去极化电流脉冲，5-HT神经元显示出缓慢而规则的放电活动。即使注入更强的电流，非5-HT神经元也会以较高的频率发射。在5-HT免疫反应性棘突投射神经元和5-HT免疫反应性棘突投射神经元之间还观察到其他一些电生理特性差异。

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