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Restoration of bladder contraction by bone marrow transplantation in rats with underactive bladder

机译：骨髓移植修复膀胱功能不全大鼠的膀胱收缩

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We attempted to increase bladder contraction by bone marrow cell transplantation in rats with underactive bladder due to bladder outlet obstruction (BOO). Twelve female rats were anesthetized with halothane to create BOO. After 1 month, the urethral obstruction was removed and they were divided into a transplant group and a sham-operated group (n = 6 each). Bone marrow cells (1 x 107/0.2 mL) isolated from green fluorescent protein transgenic rats were injected into the bladder wall of the transplant group. Rats from the sham-operated group received injection of culture medium alone One month after transplantation, isovolumetric cystometry parameters and histological features of bladder were observed as well as intact control rats (n = 6). The amplitude of bladder contractions was larger and the interval between contractions was shorter in the transplant group than the sham-operated group, and there were no differences in these parameters between the transplant group and the control group. Some green fluorescent muscle layers were found in the bladder wall of the transplant group, and these layers were also labeled by anti alpha-smooth muscle actin antibody. These results suggest that transplanted bone marrow cells may improve bladder contractility by differentiating into smooth muscle-like cells.
机译：我们试图通过由于膀胱出口阻塞（BOO）而导致膀胱活动不足的大鼠的骨髓细胞移植来增加膀胱收缩。用氟烷将十二只雌性大鼠麻醉以产生BOO。 1个月后，将尿道梗阻清除，将其分为移植组和假手术组（每组n = 6）。从绿色荧光蛋白转基因大鼠中分离出的骨髓细胞（1 x 107 / 0.2 mL）被注射到移植组的膀胱壁中。假手术组的大鼠仅在移植后一个月就接受了培养基的注射，并观察了等体积膀胱测压参数和膀胱组织学特征，以及完整对照组（n = 6）。与假手术组相比，移植组的膀胱收缩幅度更大，收缩间隔更短，并且移植组与对照组之间的这些参数没有差异。在移植组的膀胱壁中发现了一些绿色荧光肌层，这些层也被抗α-平滑肌肌动蛋白抗体标记。这些结果表明，移植的骨髓细胞可分化为平滑肌样细胞，从而改善膀胱收缩能力。

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